LOCUS       sequence-128167-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a STIM1 gain-of-function mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Ma G, Wei M, He L, Liu C, Wu B, Zhang SL, Jing J, Liang X, Senes A, 
            Tan P, Li S, Sun A, Bi Y, Zhong L, Si H, Shen Y, Li M, Lee MS, Zhou 
            W, Wang J, Wang Y, Zhou Y
  TITLE     Inside-out Ca(2+) signalling prompted by STIM1 conformational 
            switch.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7826. doi: 10.1038/ncomms8826.
  PUBMED    26184105
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7826. doi"; pages: " 
            10.1038/ncomms8826"
COMMENT     Sequence Label: pCMV-TNT-CFP-STIM1-C227W
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(115..117,118..828)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(157..178)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(418..437)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     765..786
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggatgtat gcgtccgtct tgccctgtgg ctcctctggg gactcctcct gcaccagggc
       61 cagagcctca gccatagtca cagtgagaag gcgacaggaa ccagctcggg cgccgtgagc
      121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      181 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      301 accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      421 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      541 tacaacgcca tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
      601 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      721 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggcgccaac
      841 tctga
//
