LOCUS       sequence-128169-        3083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an omega1 or omega1R vector to 
            any alpha level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1omega2_SF (GB0466)
FEATURES             Location/Qualifiers
     source          1..3083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    172..196
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(287..875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(367..386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1046..1837)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2481..2916
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3043..3065
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagtctcaa tggtaacttt actctttatt taaccataca ttttttttta tttttttcac
       61 tttgttcttc atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg
      121 ttcttcatcc accattgttc ttcatcaatc atttcgcttg agaccgatat ctgacaggat
      181 atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag
      241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      721 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaagaagag
      781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1021 tatatgtgta acattggtct agtgattatt tgccgactac cttggtgatc tcgcctttca
     1081 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     1141 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     1201 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     1261 tgcgggacaa cgtaagcact acatttcgct catcaccagc ccagtcgggc ggcgagttcc
     1321 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     1381 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     1441 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     1501 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     1561 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     1621 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     1681 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     1741 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     1801 gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc
     1861 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     1921 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac
     1981 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     2041 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
     2101 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     2161 gcgaagtcga ggcatttctg tcctggctgg aacacccctt gtattactgt ttatgtaagc
     2221 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     2281 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     2341 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     2401 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
     2461 tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggctt ttttatcccc
     2521 ggaagcctgt ggatagaggg tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt
     2581 gattcacggg gctttccggc ccgctccaaa aactatccac gtgaaatcgc taatcagggt
     2641 acgtgaaatc gctaatcgga gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat
     2701 caaaaaggca cgtgagaacg ctaatagccc tttcagatca acagcttgca aacacccctc
     2761 gctccggcaa gtagttacag caagtagtat gttcaattag cttttcaatt atgaatatat
     2821 atatcaatta ttggtcgccc ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc
     2881 gtggacaacc gcaagcggtt gcccaccgtc gagcgccttt gcccacaacc cggcggccgg
     2941 ccgcaacaga tcgttttata aatttttttt tttgaaaaag aaaaagcccg aaaggcggca
     3001 acctctcggg cttctggatt tccgatcccc ggaattagat cttggcagga tatattgtgg
     3061 tgtaacgata tcggtctcag tca
//
