LOCUS       sequence-12817-e       13284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of human C-MYC, LIN28 and 
            NANOG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 13284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hMLN
FEATURES             Location/Qualifiers
     source          1..13284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..1332
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             1357..1422
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2077..2142
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    3135..3723
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3188..3208)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3606..3617)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3813..3832)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3878..3933
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4294..4310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4294..4310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4307..4329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4318..4334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4342..4348,4349..4366,4367..4372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4387..4408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4497..4530
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4991..6916
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    7084..7117
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      7304..9093
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9635..9652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9806..10394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9886..9905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10565..11356,11357..11425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11188..11207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11426..11530)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        11561..11940
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11942..12218
                     /label=chicken beta-actin promoter
     intron          12219..13235
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     13159..13181
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     13243..13262
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 aattcgccct tcaccatgcc cctcaacgtt agcttcacca acaggaacta tgacctcgac
       61 tacgactcgg tgcagccgta tttctactgc gacgaggagg agaacttcta ccagcagcag
      121 cagcagagcg agctgcagcc cccggcgccc agcgaggata tctggaagaa attcgagctg
      181 ctgcccaccc cgcccctgtc ccctagccgc cgctccgggc tctgctcgcc ctcctacgtt
      241 gcggtcacac ccttctccct tcggggagac aacgacggcg gtggcgggag cttctccacg
      301 gccgaccagc tggagatggt gaccgagctg ctgggaggag acatggtgaa ccagagtttc
      361 atctgcgacc cggacgacga gaccttcatc aaaaacatca tcatccagga ctgtatgtgg
      421 agcggcttct cggccgccgc caagctcgtc tcagagaagc tggcctccta ccaggctgcg
      481 cgcaaagaca gcggcagccc gaaccccgcc cgcggccaca gcgtctgctc cacctccagc
      541 ttgtacctgc aggatctgag cgccgccgcc tcagagtgca tcgacccctc ggtggtcttc
      601 ccctaccctc tcaacgacag cagctcgccc aagtcctgcg cctcgcaaga ctccagcgcc
      661 ttctctccgt cctcggattc tctgctctcc tcgacggagt cctccccgca gggcagcccc
      721 gagcccctgg tgctccatga ggagacaccg cccaccacca gcagcgactc tgaggaggaa
      781 caagaagatg aggaagaaat cgatgttgtt tctgtggaaa agaggcaggc tcctggcaaa
      841 aggtcagagt ctggatcacc ttctgctgga ggccacagca aacctcctca cagcccactg
      901 gtcctcaaga ggtgccacgt ctccacacat cagcacaact acgcagcgcc tccctccact
      961 cggaaggact atcctgctgc caagagggtc aagttggaca gtgtcagagt cctgagacag
     1021 atcagcaaca accgaaaatg caccagcccc aggtcctcgg acaccgagga gaatgtcaag
     1081 aggcgaacac acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt
     1141 gccctgcgtg accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc
     1201 cttaaaaaag ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct
     1261 gaagaggact tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg
     1321 aactcttgtg cgggggatct caaaattgtc gctcctgtca aacaaactct taactttgat
     1381 ttactcaaac tggctgggga tgtagaaagc aatccaggtc cactcatggg ctccgtgtcc
     1441 aaccagcagt ttgcaggtgg ctgcgccaag gcggcagaag aggcgcccga ggaggcgccg
     1501 gaggacgcgg cccgggcggc ggacgagcct cagctgctgc acggtgcggg catctgtaag
     1561 tggttcaacg tgcgcatggg gttcggcttc ctgtccatga ccgcccgcgc cggggtcgcg
     1621 ctcgaccccc cagtggatgt ctttgtgcac cagagtaagc tgcacatgga agggttccgg
     1681 agcttgaagg agggtgaggc agtggagttc acctttaaga agtcagccaa gggtctggaa
     1741 tccatccgtg tcaccggacc tggtggagta ttctgtattg ggagtgagag gcggccaaaa
     1801 ggaaagagca tgcagaagcg cagatcaaaa ggagacaggt gctacaactg tggaggtcta
     1861 gatcatcatg ccaaggaatg caagctgcca ccccagccca agaagtgcca cttctgccag
     1921 agcatcagcc atatggtagc ctcatgtccg ctgaaggccc agcagggccc tagtgcacag
     1981 ggaaagccaa cctactttcg agaggaagaa gaagaaatcc acagccctac cctgctcccg
     2041 gaggcacaga atggggatct caaaattgtc gctcctgtca aacaaactct taactttgat
     2101 ttactcaaac tggctgggga tgtagaaagc aatccaggtc cactcatgaa caattcgccc
     2161 ttcaccatga gtgtggatcc agcttgtccc caaagcttgc cttgctttga agcatccgac
     2221 tgtaaagaat cttcacctat gcctgtgatt tgtgggcctg aagaaaacta tccatccttg
     2281 caaatgtctt ctgctgagat gcctcacacg gagactgtct ctcctcttcc ctcctccatg
     2341 gatctgctta ttcaggacag ccctgattct tccaccagtc ccaaaggcaa acaacccact
     2401 tctgcagaga atagtgtcgc aaaaaaggaa gacaaggtcc cagtcaagaa acagaagacc
     2461 agaactgtgt tctcttccac ccagctgtgt gtactcaatg atagatttca gagacagaaa
     2521 tacctcagcc tccagcagat gcaagaactc tccaacatcc tgaacctcag ctacaaacag
     2581 gtgaagacct ggttccagaa ccagagaatg aaatctaaga ggtggcagaa aaacaactgg
     2641 ccgaagaata gcaatggtgt gacgcagaag gcctcagcac ctacctaccc cagcctctac
     2701 tcttcctacc accagggatg cctggtgaac ccgactggga accttccaat gtggagcaac
     2761 cagacctgga acaattcaac ctggagcaac cagacccaga acatccagtc ctggagcaac
     2821 cactcctgga acactcagac ctggtgcacc caatcctgga acaatcaggc ctggaacagt
     2881 cccttctata actgtggaga ggaatctctg cagtcctgca tgcagttcca gccaaattct
     2941 cctgccagtg acttggaggc tgctttggaa gctgctgggg aaggccttaa tgtaatacag
     3001 cagaccacta ggtattttag tactccacaa accatggatt tattcctaaa ctactccatg
     3061 aacatgcaac ctgaagacgt gtgaaagggc gaattgcctg caggaattca agcttcgggg
     3121 actagtcata tgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3181 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3241 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3301 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3361 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3421 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3481 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     3541 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3601 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3661 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3721 cgcatcggta aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     3781 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     3841 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     3901 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     3961 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     4021 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     4081 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     4141 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     4201 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     4261 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4321 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4381 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4441 gggaaacctg tcgtgccagc ggatctcaat tccgatcata ttcaataacc cttaatataa
     4501 cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca
     4561 agcttaggat caattctcat gtttgacagc ttatcatcga taagctgatc ctcacaggcc
     4621 gcacccagct tttcttccgt tgccccagta gcatctctgt ctggtgacct tgaagaggaa
     4681 gaggaggggt cccgagaatc cccatcccta ccgtccagca aaaaggggga cgaggaattt
     4741 gaggcctggc ttgaggctca ggacgcaaat cttgaggatg ttcagcggga gttttccggg
     4801 ctgcgagtaa ttggtgatga ggacgaggat ggttcggagg atggggaatt ttcagacctg
     4861 gatctgtctg acagcgacca tgaaggggat gagggtgggg gggctgttgg agggggcagg
     4921 agtctgcact ccctgtattc actgagcgtc gtctaataaa gatgtctatt gatctctttt
     4981 agtgtgaatc atgtctgacg aggggccagg tacaggacct ggaaatggcc taggagagaa
     5041 gggagacaca tctggaccag aaggctccgg cggcagtgga cctcaaagaa gagggggtga
     5101 taaccatgga cgaggacggg gaagaggacg aggacgagga ggcggaagac caggagcccc
     5161 gggcggctca ggatcagggc caagacatag agatggtgtc cggagacccc aaaaacgtcc
     5221 aagttgcatt ggctgcaaag ggacccacgg tggaacagga gcaggagcag gagcgggagg
     5281 ggcaggagca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
     5341 ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg
     5401 aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc
     5461 aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggaggag gggcaggagc
     5521 aggaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg
     5581 agcaggagga ggggcaggag caggaggggc aggaggggca ggaggggcag gagcaggagg
     5641 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc
     5701 aggaggggca ggagcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     5761 ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggaggag gggcaggagg
     5821 ggcaggagca ggaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
     5881 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc
     5941 aggagcagga ggggcaggag caggaggtgg aggccggggt cgaggaggca gtggaggccg
     6001 gggtcgagga ggtagtggag gccggggtcg aggaggtagt ggaggccgcc ggggtagagg
     6061 acgtgaaaga gccagggggg gaagtcgtga aagagccagg gggagaggtc gtggacgtgg
     6121 agaaaagagg cccaggagtc ccagtagtca gtcatcatca tccgggtctc caccgcgcag
     6181 gccccctcca ggtagaaggc catttttcca ccctgtaggg gaagccgatt attttgaata
     6241 ccaccaagaa ggtggcccag atggtgagcc tgacgtgccc ccgggagcga tagagcaggg
     6301 ccccgcagat gacccaggag aaggcccaag cactggaccc cggggtcagg gtgatggagg
     6361 caggcgcaaa aaaggagggt ggtttggaaa gcatcgtggt caaggaggtt ccaacccgaa
     6421 atttgagaac attgcagaag gtttaagagc tctcctggct aggagtcacg tagaaaggac
     6481 taccgacgaa ggaacttggg tcgccggtgt gttcgtatat ggaggtagta agacctccct
     6541 ttacaaccta aggcgaggaa ctgcccttgc tattccacaa tgtcgtctta caccattgag
     6601 tcgtctcccc tttggaatgg cccctggacc cggcccacaa cctggcccgc taagggagtc
     6661 cattgtctgt tatttcatgg tctttttaca aactcatata tttgctgagg ttttgaagga
     6721 tgcgattaag gaccttgtta tgacaaagcc cgctcctacc tgcaatatca gggtgactgt
     6781 gtgcagcttt gacgatggag tagatttgcc tccctggttt ccacctatgg tggaaggggc
     6841 tgccgcggag ggtgatgacg gagatgacgg agatgaagga ggtgatggag atgagggtga
     6901 ggaagggcag gagtgatgta acttgttagg agacgccctc aatcgtatta aaagccgtgt
     6961 attcccccgc actaaagaat aaatccccag tagacatcat gcgtgctgtt ggtgtatttc
     7021 tggccatctg tcttgtcacc attttcgtcc tcccaacatg gggcaattgc cggaaccctt
     7081 aatataactt cgtataatgt atgctatacg aagttattag gtccctcgaa gaggttcact
     7141 agcggatctc aattgggcat acccatgttg tcacgtcact cagctccgcg ctcaacacct
     7201 tctcgcgttg gaaaacatta gcgacattta cctggtgagc aatcagacat gcgacggctt
     7261 tagcctggcc tccttaaatt cacctaagaa tgggagcaac cagcaggaaa aggacaagca
     7321 gcgaaaattc acgccccctt gggaggtggc ggcatatgca aaggatagca ctcccactct
     7381 actactgggt atcatatgct gactgtatat gcatgaggat agcatatgct acccggatac
     7441 agattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
     7501 agattaggat agcctatgct acccagatat aaattaggat agcatatact acccagatat
     7561 agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
     7621 agattaggat agcatatgct acccagatat agattaggat agcatatgct atccagatat
     7681 ttgggtagta tatgctaccc agatataaat taggatagca tatactaccc taatctctat
     7741 taggatagca tatgctaccc ggatacagat taggatagca tatactaccc agatatagat
     7801 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat
     7861 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
     7921 taggatagcc tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
     7981 gtagtatatg ctacccatgg caacattagc ccaccgtgct ctcagcgacc tcgtgaatat
     8041 gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga
     8101 tcgcagcaat cgcgccccta tcttggcccg cccacctact tatgcaggta ttccccgggg
     8161 tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt ggttagcggg gttacaatca
     8221 gccaagttat tacaccctta ttttacagtc caaaaccgca gggcggcgtg tgggggctga
     8281 cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc
     8341 cccattggcg tggagccccg tttaattttc gggggtgtta gagacaacca gtggagtccg
     8401 ctgctgtcgg cgtccactct ctttcccctt gttacaaata gagtgtaaca acatggttca
     8461 cctgtcttgg tccctgcctg ggacacatct taataacccc agtatcatat tgcactagga
     8521 ttatgtgttg cccatagcca taaattcgtg tgagatggac atccagtctt tacggcttgt
     8581 ccccacccca tggatttcta ttgttaaaga tattcagaat gtttcattcc tacactagta
     8641 tttattgccc aaggggtttg tgagggttat attggtgtca tagcacaatg ccaccactga
     8701 accccccgtc caaattttat tctgggggcg tcacctgaaa ccttgttttc gagcacctca
     8761 catacacctt actgttcaca actcagcagt tattctatta gctaaacgaa ggagaatgaa
     8821 gaagcaggcg aagattcagg agagttcact gcccgctcct tgatcttcag ccactgccct
     8881 tgtgactaaa atggttcact accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga
     8941 cagctcatgg ggtgggagat atcgctgttc cttaggaccc ttttactaac cctaattcga
     9001 tagcatatgc ttcccgttgg gtaacatatg ctattgaatt agggttagtc tggatagtat
     9061 atactactac ccgggaagca tatgctaccc gtttagggtt aacaaggggg ccttataaac
     9121 actattgcta atgccctctt gagggtccgc ttatcggtag ctacacaggc ccctctgatt
     9181 gacgttggtg tagcctcccg tagtcttcct gggcccctgg gaggtacatg tcccccagca
     9241 ttggtgtaag agcttcagcc aagagttaca cataaaggca atgttgtgtt gcagtccaca
     9301 gactgcaaag tctgctccag gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt
     9361 tataaggatg tcaactacag tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca
     9421 tgtggaacag ggcccagttg gcaagttgta ccaaccaact gaagggatta catgcactgc
     9481 cccgcgaaga aggggcagag atgtcgtagt caggtttagt tcgtccgggg cggggcatcg
     9541 atcctctaga gtcgacgcta gcggatccgc tgcattaatg aatcggccaa cgcgcgggga
     9601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11521 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt
    11581 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    11641 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    11701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    11761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    11821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    11881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    11941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
    12001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
    12061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcgggcgagg cggagaggtg
    12121 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
    12181 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
    12241 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
    12301 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
    12361 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
    12421 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
    12481 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
    12541 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
    12601 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
    12661 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
    12721 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
    12781 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
    12841 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
    12901 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
    12961 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
    13021 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
    13081 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
    13141 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
    13201 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
    13261 tgctgtctca tcattttggc aaag
//
