LOCUS       sequence-128172-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Martinez-Cruz LA, Dreyer MK, Boisvert DC, Yokota H, Martinez-Chantar
            ML, Kim R, Kim SH
  TITLE     Crystal structure of MJ1247 protein from M. jannaschii at 2.0 A 
            resolution infers a molecular function of 3-hexulose-6-phosphate 
            isomerase.
  JOURNAL   Structure. 2002 Feb . 10(2):195-204.
  PUBMED    11839305
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2002 Feb . 10(2):195-204."
COMMENT     Sequence Label: pET21a.MJ1247
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             736..753
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata catatggtgt cgaaattgga agaacttgat atagtatcta
      181 acaatatatt gatattaaaa aaattctata caaacgatga gtggaaaaat aaactggatt
      241 ctttaattga taggattata aaagctaaaa aaatttttat ttttggagta ggtaggagtg
      301 gatatattgg aagatgtttt gccatgagat taatgcatct tggttttaaa tcatattttg
      361 ttggggaaac tacaactcct tcctatgaaa aagatgattt actaatttta atatcgggta
      421 gtggaagaac agagagtgtt ttaacagtag ctaaaaaggc aaagaatata aataacaaca
      481 ttatcgcaat tgtatgtgaa tgtggaaatg tagtagagtt tgctgattta acaattcctt
      541 tagaagtgaa gaaatcaaaa tatttaccaa tgggaaccac ttttgaagaa acagctttga
      601 tatttttaga tttagtcata gctgagatta tgaaaagatt gaatttagat gaaagtgaaa
      661 taataaagag acattgtaat ttactttgag gatccgaatt cgagctccgt cgacaagctt
      721 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      781 aaggaagctg agttggctgc tgcc
//
