LOCUS       sequence-128187-         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC2,cisRaj12-sfGFP, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKbreak12
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        76..110
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     protein_bind    365..381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             428..439
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gggtttcccg tgctcagtat ctctatcact gatagggatg tcaatctcta tcactgatag
       61 ggatctagaa tcgatttgac agctagctca gtcctaggta taatgctagc ggcgctgcct
      121 tcgggcgacc ctgatgagct tgagtttagc tcgtcactgt ccaggttcaa tcaggcgaaa
      181 cggtgaaagc cgtaggttgc ccgacgggca ggaagaaggg ttcctttgag cgaatctagc
      241 ggcacctcgc taggatttgc tcgaagggat tctgggcaaa gcccgccgaa aggcgggctt
      301 ttctgtacta gtctgcaggg atcgacgaga gcatgcatat aaatgtgagc ggataacatt
      361 gacattgtga gcggataaca agatactgag cacacccagt atcattctct tcttcctgcc
      421 cacgcggaaa gaggagaaag gtgtaatgac tagtcgtaaa ggcgaagagc tgttcactgg
      481 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      541 tggcgag
//
