LOCUS       sequence-128188-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj11
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    52..73
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    316..334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    341..359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    427..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             490..501
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gagccagtga gttgattgct acgtaattag ttagttagcc cttagtgact cgaattcgcg
       61 gccgcttcta gagcgctgct ctcgtcgatc ccttaagcgc cggcgaagat ctcacagaaa
      121 agcccgcctt tcggcgggct ttggcctcgc ataaatctgt cacagcatca acgtttccgc
      181 tgaactgtga cagactcaca caatcaaccc tccccggtgg aatccaggac acctgccaag
      241 ggcctttcgg ctggtatgta tttcgtccta tttgggctca tcagctggat gtacccggga
      301 aagaagtgct cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggat
      361 acgtacgatc gggtgcgagc ggatcgagat gcatgctagc ataaatgtga gcggataaca
      421 ttgacattgt gagcggataa caagatactg agcaccctcg cataatttca cttcttcaat
      481 cctcccgtta aagaggagaa attatgaatg actagccgta aaggcgaaga gctgttcact
      541 ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg
      601 cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctga
//
