LOCUS       sequence-128189-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj12-olsa
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    62..83
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    288..306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             439..450
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ccgtgaaggt gagccagtga gttgattgct acgtaattag ttagttagcc cttagtgact
       61 cgaattcgcg gccgcttcta gagacagaaa agcccgcctt tcggcgggct ttgcccagaa
      121 tcccttcgag caaatcctag cgaggtgccg ctagattcgc tcaaaggaac ccttcttcct
      181 gcccgtctgg aatccaggac acctgccaag ggcctttcgg ctggtatgta tttcgtccta
      241 tttgggactc atcagctgga tgtacgaagg cagcgccgtg ctcagtatct ctatcactga
      301 tagggatgtc aatctctatc actgataggg agctagccaa ttgatgcata taaatgtgag
      361 cggataacat tgacattgtg agcggataac aagatactga gcaccaattc taccattcac
      421 ctcttggatt tgggtattaa agaggagaaa ggtaccatga ctagtcgtaa aggcgaagag
      481 ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag
      541 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
      601 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
      661 ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc
      721 gccatgccgg aaggctatgt gcaggaacgc acgatt
//
