LOCUS       sequence-128193-        2796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a STIM1 gain-of-function mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma G, Wei M, He L, Liu C, Wu B, Zhang SL, Jing J, Liang X, Senes A, 
            Tan P, Li S, Sun A, Bi Y, Zhong L, Si H, Shen Y, Li M, Lee MS, Zhou 
            W, Wang J, Wang Y, Zhou Y
  TITLE     Inside-out Ca(2+) signalling prompted by STIM1 conformational 
            switch.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7826. doi: 10.1038/ncomms8826.
  PUBMED    26184105
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7826. doi"; pages: " 
            10.1038/ncomms8826"
COMMENT     Sequence Label: pCMV-TNT-CFP-STIM1-C227W
FEATURES             Location/Qualifiers
     source          1..2796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(121..123,124..834)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(163..184)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(424..443)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     771..792
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaattcatgg atgtatgcgt ccgtcttgcc ctgtggctcc tctggggact cctcctgcac
       61 cagggccaga gcctcagcca tagtcacagt gagaaggcga caggaaccag ctcgggcgcc
      121 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      181 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      241 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      301 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
      361 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      421 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      481 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      541 ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
      601 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      661 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      721 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      781 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccggc
      841 gccaactctg aggagtccac tgcagcagag ttttgccgaa ttgacaagcc cctgtgtcac
      901 agtgaggatg agaaactcag cttcgaggca gtccgtaaca tccacaaact gatggacgat
      961 gatgccaatg gtgatgtgga tgtggaagaa agtgatgagt tcctgaggga agacctcaat
     1021 taccatgacc caacagtgaa acacagcacc ttccatggtg aggataagct catcagcgtg
     1081 gaggacctgt ggaaggcatg gaagtcatca gaagtataca attggaccgt ggatgaggtg
     1141 gtacagtggc tgatcacata tgtggagctg cctcagtatg aggagacctt ccggaagctg
     1201 cagctcagtg gccatgccat gccaaggctg gctgtcacca acaccaccat gacagggact
     1261 gtgctgaaga tgacagaccg gagtcatcgg cagaagctgc agctgaaggc tctggataca
     1321 gtgctctttg ggcctcctct cttgactcgc cataatcacc tcaaggactt catgctggtg
     1381 gtgtctatcg ttattggtgt gggcggctgg tggtttgcct atatccagaa ccgttactcc
     1441 aaggagcaca tgaagaagat gatgaaggac ttggaggggt tacaccgagc tgagcagagt
     1501 ctgcatgacc ttcaggaaag gctgcacaag gcccaggagg agcaccgcac agtggaggtg
     1561 gagaaggtcc atctggaaaa gaagctgcgc gatgagatca accttgctaa gcaggaagcc
     1621 cagcggctga aggagctgcg ggagggtact gagaatgagc ggagccgcca aaaatatgct
     1681 gaggaggagt tggagcaggt tcgggaggcc ttgaggaaag cagagaagga gctagaatct
     1741 cacagctcat ggtatgctcc agaggccctt cagaagtggc tgcagctgac acatgaggtg
     1801 gaggtgcaat attacaacat caagaagcaa aatgctgaga agcagctgct ggtggccaag
     1861 gagggggctg agaagataaa aaagaagaga aacacactct ttggcacctt ccacgtggcc
     1921 cacagctctt ccctggatga tgtagatcat aaaattctaa cagctaagca agcactgagc
     1981 gaggtgacag cagcattgcg ggagcgcctg caccgctggc aacagatcga gatcctctgt
     2041 ggcttccaga ttgtcaacaa ccctggcatc cactcactgg tggctgccct caacatagac
     2101 cccagctgga tgggcagtac acgccccaac cctgctcact tcatcatgac tgacgacgtg
     2161 gatgacatgg atgaggagat tgtgtctccc ttgtccatgc agtcccctag cctgcagagc
     2221 agtgttcggc agcgcctgac ggagccacag catggcctgg gatctcagag ggatttgacc
     2281 cattccgatt cggagtcctc cctccacatg agtgaccgcc agcgtgtggc ccccaaacct
     2341 cctcagatga gccgtgctgc agacgaggct ctcaatgcca tgacttccaa tggcagccac
     2401 cggctgatcg agggggtcca cccagggtct ctggtggaga aactgcctga cagccctgcc
     2461 ctggccaaga aggcattact ggcgctgaac catgggctgg acaaggccca cagcctgatg
     2521 gagctgagcc cctcagcccc acctggtggc tctccacatt tggattcttc ccgttctcac
     2581 agccccagct ccccagaccc agacacacca tctccagttg gggacagccg agccctgcaa
     2641 gccagccgaa acacacgcat tccccacctg gctggcaaga aggctgtggc tgaggaggat
     2701 aatggctcta ttggcgagga aacagactcc agcccaggcc ggaagaagtt tcccctcaaa
     2761 atctttaaga agcctcttaa gaagtaggcg gccgct
//
