LOCUS       sequence-128227-        2163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Cyan Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Cyan Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..519)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1828..1905
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggggt accaaggtgt acgaccccga gcagaggaag
      721 aggatgatca ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc
      781 ttcatcaact actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc
      841 aacgccacta gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg
      901 tgcatcatcc ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac
      961 aggctgctgg accactacaa gtacctgacc gcctggttcg agcttctgaa cctgcccaag
     1021 aagatcatct tcgtgggcca cgactggggc gccgccctgg ccttccacta cgcctacgag
     1081 caccaggaca ggatcaaggc catcgtgcac atggagagcg tggtggacgt gatcgagagc
     1141 tgggacgagt ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag
     1201 aagatggtgc tggagaacaa cttcttcgtg gagaccgtgc tgcccagcaa gatcatgaga
     1261 aagctggagc ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg
     1321 agaagaccca ccctgagctg gcccagagag atccccctgg tgaagggccc atggatgcat
     1381 gaccaactga cagaagagca gattgcagag ttcaaagaag ccttctcatt attcgacaag
     1441 gatggggacg gcaccatcac cacaaaggaa cttggcaccg ttatgaggtc gcttggacaa
     1501 aacccaacgg aagcagaatt gcaggatatg atcaatgaag tcgatgctga tggcaatgga
     1561 acgatttact ttcctgaatt tcttactatg atggctagaa aaatgaagga cacagacagc
     1621 gaagaggaaa tccgagaagc attccgtgtt tttgacaagg atgggaacgg ctacatcagc
     1681 gctgctcagt tacgtcacgt catgacaaac ctcggggaga agttaacaga tgaagaagtt
     1741 gatgaaatga taagggaagc agatatcgat ggtgatggcc aagtaaacta tgaagagttt
     1801 gtacaaatga tgacagcaaa gggggggaag aggcgctgga agaaaaactt cattgccgtc
     1861 agcgctgcca accggttcaa gaagatctcc agctccgggg cactggagct cggcaagccc
     1921 gacgtggtgc agatcgtgag aaactacaac gcctacctga gagccagcga cgacctgccc
     1981 aagctgttca tcgagggtga ccccggcttc ttcagcaacg ccatcgtgga gggcgccaag
     2041 aagttcccca acaccgagtt cgtgaaggtg aagggcctgc acttcctcca ggaggacgcc
     2101 cccgacgaga tgggcaagta catcaagagc ttcgtggaga gagtgctgaa gaacgagcag
     2161 taa
//
