LOCUS       sequence-128228-        2277 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(118..120,121..633)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(160..181)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(421..440)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     768..789
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1942..2019
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atgctgttca acctgaggat cctgctgaac aacgccgcct tcaggaacgg ccacaacttc
       61 atggtgagga acttcaggtg cagccagccc ctgcagaaca aggtgcaggg ggaattcgtg
      121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      181 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      301 accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      541 gagtacaact actttagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
      601 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
      661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      721 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      781 gagttcgtga ccgccgccgg gggtaccaag gtgtacgacc ccgagcagag gaagaggatg
      841 atcaccggcc cccagtggtg ggccaggtgc aagcagatga acgtgctgga cagcttcatc
      901 aactactacg acagcgagaa gcacgccgag aacgccgtga tcttcctgca cggcaacgcc
      961 actagcagct acctgtggag gcacgtggtg ccccacatcg agcccgtggc caggtgcatc
     1021 atccccgatc tgatcggcat gggcaagagc ggcaagagcg gcaacggcag ctacaggctg
     1081 ctggaccact acaagtacct gaccgcctgg ttcgagcttc tgaacctgcc caagaagatc
     1141 atcttcgtgg gccacgactg gggcgccgcc ctggccttcc actacgccta cgagcaccag
     1201 gacaggatca aggccatcgt gcacatggag agcgtggtgg acgtgatcga gagctgggac
     1261 gagtggccag acatcgagga ggacatcgcc ctgatcaaga gcgaggaggg cgagaagatg
     1321 gtgctggaga acaacttctt cgtggagacc gtgctgccca gcaagatcat gagaaagctg
     1381 gagcccgagg agttcgccgc ctacctggag cccttcaagg agaagggcga ggtgagaaga
     1441 cccaccctga gctggcccag agagatcccc ctggtgaagg gcccatggat gcatgaccaa
     1501 ctgacagaag agcagattgc agagttcaaa gaagccttct cattattcga caaggatggg
     1561 gacggcacca tcaccacaaa ggaacttggc accgttatga ggtcgcttgg acaaaaccca
     1621 acggaagcag aattgcagga tatgatcaat gaagtcgatg ctgatggcaa tggaacgatt
     1681 tactttcctg aatttcttac tatgatggct agaaaaatga aggacacaga cagcgaagag
     1741 gaaatccgag aagcattccg tgtttttgac aaggatggga acggctacat cagcgctgct
     1801 cagttacgtc acgtcatgac aaacctcggg gagaagttaa cagatgaaga agttgatgaa
     1861 atgataaggg aagcagatat cgatggtgat ggccaagtaa actatgaaga gtttgtacaa
     1921 atgatgacag caaagggggg gaagaggcgc tggaagaaaa acttcattgc cgtcagcgct
     1981 gccaaccggt tcaagaagat ctccagctcc ggggcactgg agctcggcaa gcccgacgtg
     2041 gtgcagatcg tgagaaacta caacgcctac ctgagagcca gcgacgacct gcccaagctg
     2101 ttcatcgagg gtgaccccgg cttcttcagc aacgccatcg tggagggcgc caagaagttc
     2161 cccaacaccg agttcgtgaa ggtgaagggc ctgcacttcc tccaggagga cgcccccgac
     2221 gagatgggca agtacatcaa gagcttcgtg gagagagtgc tgaagaacga gcagtaa
//
