LOCUS       sequence-128229-        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Orange Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Orange Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1..654
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             1795..1872
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggtgagcg tgatcaagcc cgagatgaag atgaggtact acatggacgg ctccgtcaat
       61 gggcatgagt tcacaatcga gggtgagggc acaggcagac cttacgaggg acatcaggag
      121 atgacactgc gcgtcacaat ggccgagggc gggccaatgc ctttcgcctt cgacctggtg
      181 tcccacgtgt tctgttacgg ccacagagtt tttactaaat atccagaaga gatcccagac
      241 tatttcaagc aggcctttcc tgagggcctg tcctgggaga ggtccctgga gttcgaggac
      301 ggcggctccg cctccgtgag cgcccacatc agcctgaggg gcaacacctt ctaccacaag
      361 tccaagttca ccggcgtgaa cttccccgcc gacggcccca tcatgcagaa ccagagcgtg
      421 gactgggagc cctccaccga gaagatcacc gccagcgacg gcgtgctgaa gggcgacgtg
      481 accatgtacc tgaagctgga gggcggcggc aaccacaagt gccagatgaa gaccacctac
      541 aaggccgcca aggagatcct ggagatgccc ggcgaccact acatcggcca caggctggtg
      601 aggaagaccg agggcaacat caccgagcag gtggaggacg ccgtggccca ctccggtacc
      661 aaggtgtacg accccgagca acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc
      721 tgcaagcaaa tgaacgtgct ggactccttc atcaactact atgattccga gaagcacgcc
      781 gagaacgccg tgatttttct gcatggtaac gctacctcca gctacctgtg gaggcacgtc
      841 gtgcctcaca tcgagcccgt ggctagatgc atcatccctg atctgatcgg aatgggtaag
      901 tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc actacaagta cctcaccgct
      961 tggttcgagc tgctgaacct tccaaagaaa atcatctttg tgggccacga ctgggggagc
     1021 gctctggcct ttcactacgc ctacgagcac caagacagga tcaaggccat cgtccatatg
     1081 gagagtgtcg tggacgtgat cgagtcctgg atggggtggc ctgacatcga ggaggagctg
     1141 gccctgatca agagcgaaga gggcgagaaa atggtgcttg agaataactt cttcgtcgag
     1201 accctgttgc caagcaagat catgcggaaa ctggagcctg aggagttcgc tgcctacctg
     1261 gagccattca aggagaaggg cgaggttaga cggcctaccc tctcctggcc tcgcgagatc
     1321 cctctcgtta agggaccatg gatgcatgac caactgacag aagagcagat tgcagagttc
     1381 aaagaagcct tctcattatt cgacaaggat ggggacggca ccatcaccac aaaggaactt
     1441 ggcaccgtta tgaggtcgct tggacaaaac ccaacggaag cagaattgca ggatatgatc
     1501 aatgaagtcg atgctgatgg caatggaacg atttactttc ctgaatttct tactatgatg
     1561 gctagaaaaa tgaaggacac agacagcgaa gaggaaatcc gagaagcatt ccgtgttttt
     1621 gacaaggatg ggaacggcta catcagcgct gctcagttac gtcacgtcat gacaaacctc
     1681 ggggagaagt taacagatga agaagttgat gaaatgataa gggaagcaga tatcgatggt
     1741 gatggccaag taaactatga agagtttgta caaatgatga cagcaaaggg ggggaagagg
     1801 cgctggaaga aaaacttcat tgccgtcagc gctgccaacc ggttcaagaa gatctccagc
     1861 tccggggcac tggagctcgg caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc
     1921 taccttcggg ccagcgacga tctgcctaag ctgttcatcg agtccgaccc tgggttcttt
     1981 tccaacgcta ttgtcgaggg agctaagaag ttccctaaca ccgagttcgt gaaggtgaag
     2041 ggcctccact tcctccagga ggacgctcca gatgaaatgg gtaagtacat caagagcttc
     2101 gtggagcgcg tgctgaagaa cgagcagtaa
//
