LOCUS       sequence-128234-       11178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral vector for the expression of the Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: Adeno Cas9
FEATURES             Location/Qualifiers
     source          1..11178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..258
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10..30
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     181..200
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        277..352
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        449..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        736..1013
                     /label=chicken beta-actin promoter
     intron          1014..1241
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1253..1262
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1262..1327
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1334..1354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1379..5479
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1379..5479
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5480..5527
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5550..5566)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5550..5566)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    5586..5720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5610..5629)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5664..5683
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5863..5885)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      complement(8960..9548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9040..9059)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9719..10510,10511..10579))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10342..10361
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10580..10684)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10752..10770
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     repeat_region   10798..10900
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10988..11138
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 ggcgcatgtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
       61 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
      121 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      181 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      241 tggaaaggac gaaacaccgg gtcttcgaga agacctgttt tagagctaga aatagcaagt
      301 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt
      361 tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat
      421 tctgcagaca aatggctcta gaggtacccg ttacataact tacggtaaat ggcccgcctg
      481 gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      781 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      841 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
      901 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      961 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1021 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     1081 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     1141 gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg gtattaatgt
     1201 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
     1261 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
     1321 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
     1381 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
     1441 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
     1501 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
     1561 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     1621 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     1681 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     1741 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     1801 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     1861 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     1921 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     1981 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     2041 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
     2101 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
     2161 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     2221 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     2281 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     2341 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     2401 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     2461 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     2521 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     2581 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     2641 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     2701 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     2761 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     2821 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     2881 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     2941 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     3001 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     3061 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     3121 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     3181 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     3241 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     3301 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     3361 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     3421 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     3481 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     3541 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     3601 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     3661 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     3721 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     3781 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     3841 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt
     3901 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     3961 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     4021 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     4081 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     4141 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     4201 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     4261 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     4321 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     4381 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     4441 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     4501 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     4561 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     4621 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     4681 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     4741 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     4801 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     4861 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     4921 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     4981 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     5041 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     5101 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     5161 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     5221 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     5281 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     5341 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     5401 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     5461 gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa
     5521 gaaaaagtaa gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
     5581 ggggagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     5641 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5701 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5761 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     5821 atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     5941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagtccccgc
     6001 ggtggcagat ctggaaggtg ctgaggtacg atgagacccg caccaggtgc agaccctgcg
     6061 agtgtggcgg taaacatatt aggaaccagc ctgtgatgct ggatgtgacc gaggagctga
     6121 ggcccgatca cttggtgctg gcctgcaccc gcgctgagtt tggctctagc gatgaagata
     6181 cagattgagg tactgaaatg tgtgggcgtg gcttaagggt gggaaagaat atataaggtg
     6241 ggggtcttat gtagttttgt atctgttttg cagcagccgc cgccgccatg agcaccaact
     6301 cgtttgatgg aagcattgtg agctcatatt tgacaacgcg catgccccca tgggccgggg
     6361 tgcgtcagaa tgtgatgggc tccagcattg atggtcgccc cgtcctgccc gcaaactcta
     6421 ctaccttgac ctacgagacc gtgtctggaa cgccgttgga gactgcagcc tccgccgccg
     6481 cttcagccgc tgcagccacc gcccgcggga ttgtgactga ctttgctttc ctgagcccgc
     6541 ttgcaagcag tgcagcttcc cgttcatccg cccgcgatga caagttgacg gctcttttgg
     6601 cacaattgga ttctttgacc cgggaactta atgtcgtttc tcagcagctg ttggatctgc
     6661 gccagcaggt ttctgccctg aaggcttcct cccctcccaa tgcggtttaa aacataaata
     6721 aaaaaccaga ctctgtttgg atttggatca agcaagtgtc ttgctgtctt tatttagggg
     6781 ttttgcgcgc gcggtaggcc cgggaccagc ggtctcggtc gttgagggtc ctgtgtattt
     6841 tttccaggac gtggtaaagg tgactctgga tgttcagata catgggcata agcccgtctc
     6901 tggggtggag gtagcaccac tgcagagctt catgctgcgg ggtggtgttg tagatgatcc
     6961 agtcgtagca ggagcgctgg gcgtggtgcc taaaaatgtc tttcagtagc aagctgattg
     7021 ccaggggcag gcccttggtg taagtgttta caaagcggtt aagctgggat gggtgcatac
     7081 gtggggatat gagatgcatc ttggactgta tttttaggtt ggctatgttc ccagccatat
     7141 ccctccgggg attcatgttg tgcagaacca ccagcacagt gtatccggtg cacttgggaa
     7201 atttgtcatg tagcttagaa ggaaatgcgt ggaagaactt ggagacgccc ttgtgacctc
     7261 caagattttc catgcattcg tccataatga tggcaatggg cccacgggcg gcggcctggg
     7321 cgaagatatt tctgggatca ctaacgtcat agttgtgttc caggatgaga tcgtcatagg
     7381 ccatttttac aaagcgcggg cggagggtgc cagactgcgg tataatggtt ccatccggcc
     7441 caggggcgta gttaccctca cagatttgca tttcccacgc tttgagttca gatgggggga
     7501 tcatgtctac ctgcggggcg atgaagaaaa cggtttccgg ggtaggggag atcagctggg
     7561 aagaaagcag gttcctgagc agctgcgact taccgcagcc ggtgggcccg taaatcacac
     7621 ctattaccgg ctgcaactgg tagttaagag agctgcagct gccgtcatcc ctgagcaggg
     7681 gggccacttc gttaagcatg tccctgactc gcatgttttc cctgaccaaa tccgccagaa
     7741 ggcgctcgcc gcccagcgat agcagttctt gcaaggaagc aaagtttttc aacggtttga
     7801 gaccgtccgc cgtaggcatg cttttgagcg tttgaccaag cagttccagg cggtcccaca
     7861 gctcggtcac ctgctctacg gcatctcgat ccagcatatc tcctcgtttc gcgggttggg
     7921 gcggctttcg ctgtacggca gtagtcggtg ctcgtccaga cgggccaggg tcatgtcttt
     7981 ccacgggcgc agggtcctcg tcagcgtagt ctgggtcacg gtgaaggggt gcgctccggg
     8041 ctgcgcgctg gccagggtgc gcttgaggct ggtcctgctg gtgctgaagc gctgccggtc
     8101 ttcgccctgc gcgtcggcca ggtagcattt gaccatggtg tcatagtcca gcccctccgc
     8161 ggcgtggccc ttggcgcgca gcttgccctt ggaggaggcg ccgcacgagg ggcagtgcag
     8221 acttttgagg gcgtagagct tgggcgcgag aaataccgat tccggggagt aggcatccgc
     8281 gccgcaggcc ccgcagacgg tctcgcattc cacgagccag gtgagctctg gccgttcggg
     8341 gtcaaaaacc aggtttcccc catgcttttt gatgcgtttc ttacctctgg tttccatgag
     8401 ccggtgtcca cgctcggtga cgaaaaggct gtccgtgtcc ccgtatacag acttgagagg
     8461 cctgtcctcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     8521 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     8581 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     8641 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     8701 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     8761 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     8821 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     8881 aggtagatga cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa
     8941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9361 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     9421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9841 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10021 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10321 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10681 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    10741 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaattaatt aagctagcat
    10801 catcaataat ataccttatt ttggattgaa gccaatatga taatgagggg gtggagtttg
    10861 tgacgtggcg cggggcgtgg gaacggggcg ggtgacgtag tagtgtggcg gaagtgtgat
    10921 gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac gtttttggtg
    10981 tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat gttgtagtaa
    11041 atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat aagaggaagt
    11101 gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg agatccggta
    11161 ccgtttaaac tcgagaat
//
