LOCUS       sequence-128252-         468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB216
FEATURES             Location/Qualifiers
     source          1..468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(72..78,79..96,97..102))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(139..263)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(289..306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(289..305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg gatcctaact
       61 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatgc
      121 ggcccctcga ggggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
      181 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      241 tcagcttttt tgtacaaact tgttgataac tctagaccca agcttggcac tggccgtcgt
      301 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      361 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      421 gttgcgcagc ctgaatggcg aatgctagaa cagcttgagc ttggatca
//
