LOCUS       sequence-128266-       13565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  proximal somatic gonad expression of DAF-16 in C.elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13565)
  AUTHORS   Qin Z, Hubbard EJ
  TITLE     Non-autonomous DAF-16/FOXO activity antagonizes age-related loss of 
            C. elegans germline stem/progenitor cells.
  JOURNAL   Nat Commun. 2015 May 11;6:7107. doi: 10.1038/ncomms8107.
  PUBMED    25960195
REFERENCE   2  (bases 1 to 13565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-05-11"; volume: "6:7107. doi"; pages: " 
            10.1038/ncomms8107"
COMMENT     Sequence Label: pGC629
FEATURES             Location/Qualifiers
     source          1..13565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9625..9641)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9626..9642)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9695..9723)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10471..10491
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     10571..10587
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10571..10587
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12926..12945)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12927..12945)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12952..12969)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12952..12968)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12961..12983)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13110..13565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13197..13216)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13407..13428
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataagca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt tcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggc agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag gggggaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggccga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctggagtac gcccaactct
     2221 agtcatttct agctcctcac caaacgaatt ttacttgaac tcaagctatt tttttgtaaa
     2281 aaaaatttac aatgagttgt gagcttctgt gaacaaggta tttttagtga aactggttaa
     2341 tgacgaatct ggaaatcttg ccgattttta agcaaacaat cagcaaggtt gaactgagta
     2401 tatactgctg aaattccttg tcttcctctt tttttcatac gtcatcccac atcatttctt
     2461 cttaatcagt cagattcata gtctcaaacc attttgtcaa aatcgcttgt ttttagcttt
     2521 cagatttagc ccaataaatt gtatgtatga gaaagaagaa aagttagaga aaactagcag
     2581 aatgagtcat taatcataac atattttttg atgttatttt tgcgcagtaa agacggaaaa
     2641 gcagtctaga atatgcacaa acaccggctc ttcgcatacg acgcatctta aaatcccttt
     2701 tcggctttgc cacgtcatta gggaaaagcg gtgatgggca tttttagaaa ttcgttttta
     2761 gtttgataat ccttaacaga gcctgcagcg aaagttttca aaaaatacat ttttaaatta
     2821 cagtcgaaag gtggtttctc agtttttatt taattttaaa aattttactt tgaaattttg
     2881 aaaaattatt atttttcttt aaattttatg ttctaattat ttttcaaata atcgtatgta
     2941 tttaaacttt gcagtggttt aaacatttgc tctgaatttt tcaaaaaact caactttttt
     3001 gtaaattggc aattaccaaa aaattaacgg aacttcaaca atttggtttg ggattttttt
     3061 agtacgatat atacatatta tatatttggt cattttaaac aaaatttgat gctacttcaa
     3121 gtaaaaaata taaggacaaa aattcctaga aatgacctac caacttttag aattattttt
     3181 gcatttcggg tgctggaacc agccggctgc caatgttttt atgttcagtt ggaaaggaag
     3241 aaggaaacca gaagttcaaa aattatagaa gtgatttaaa ttaaaaatgg catttgaaac
     3301 tgactaattt agacttctca tgaattttca aaaatgtttt aaagtttttc aatctcgttt
     3361 tcttctcttt tttagtaaaa aatcaaacag gataataaat tcccgaaaaa ccacactttt
     3421 ataaccttta taaacctcac aggcattaaa ggtcattcca caaaaaactg gtcatacaat
     3481 aaaaaacttg cagtgtggaa cagtttagaa ccgtgcgaaa aaattaaata aattgttttt
     3541 ttttgggtca aatgttttat tggggtattg ataaataaac atatttttgc gggttttcga
     3601 gattttataa attttctctc tgaaatgcct tggacaactt cctcatatga aaagattcgt
     3661 acttattttt gcatgtttgc cttctgaaag caaataataa aatctattgt gatttgattc
     3721 gttttccttc tttttacttt tcattcgtct caacgtggct ttgatctgtt tcctggcttt
     3781 aacctttttt cgaatattgt aggcccaaac gtactgacct gtttgtcgcc tacctgcctc
     3841 ctctgcctag ataccaccta ttttttgagg tttcacatat gttcaaatgt tcaatgttcg
     3901 cacagtataa atgtacacac cctctatttt ctcatttcta tcagacgaaa aattattttt
     3961 aaaaaataag cttcaagtgt acttgggtag gcgtaggcac ctttcagaca ggcatttctg
     4021 tgcctatgtg gtttattttt tacaattttc aattttttca aactccagtt ccagatcata
     4081 aaattccaag tcataaaagc atttcaatat ttccacgttt cagctgttct tttcctgaaa
     4141 tgttgcacct gaaaacattt catattgaaa attgcatctg gcatttattt tttgttgcgg
     4201 atgtgtcaaa atatattaat cctcttctta ttcttcctaa tccttatgca ccactcaaag
     4261 attcttgtcc cagttgactt taaaatctga gctctatctt actcttcatt ccataattag
     4321 ctgtattttt caaaaaagta ctaaaaatgt caaactgata gcgagagcga gtttgaaata
     4381 tatctgaaaa tgtgaaaaat attctcttgc tgttaaaata ttctagttgt tgattatttt
     4441 ccggtcaaaa tttaagattt tggtttctaa taaacaaaaa aaccttatca ctcacatatc
     4501 tttcagaaat ttctcacggc ttcctctctc tttttctccg attttctttc aaacgctaat
     4561 ccctccaaac tctcctttta tttctttctt gtgtgtgcat ttcctttttc gatcctcatc
     4621 ttaaacatca tagcattttc attttcagat caataccaat aataggaaaa tcatctgctg
     4681 ggcagctgta aaacgtcttt actccactca aaacacaggt gagtctgaaa aaatatgtat
     4741 tcgtgttttt aataataact ttaaatcaaa aaaacataac tagtgaaaag ctaaaacgct
     4801 ataacactaa aaatcaaacc ccgtggcgtc aaaagttagt ttcaatgttg atctttatgt
     4861 taactgaagt cacaccagaa cgtttgaaat tatacgcatg aaatagctca ttagaaactc
     4921 ttcaatgatc cgcttaccaa acacagaaga cgtgttttga aaacacctgt aatgagaagt
     4981 ttcccttgtg catgaagttt atgaacttta gattaagttg gaaaataata aatattgggt
     5041 ttggatttcc cggaaatagt tttggttttt tgaaaaatta gacttgattt ttgaaattgg
     5101 gcatttttct tggaacacta gacaacaaaa tttgttaaaa ttataccagt tctgaaagca
     5161 aaacacaaaa ctatgagtta caaaaaaaaa catttttcaa gtttaaatga tttgggaaca
     5221 tggaaattgt ataacttccc gtagttgtgg acaatttttt gccaaaaact taaagttcag
     5281 aagctccagg gatcgtagct ttacaaaaat tatttgcttc gatatggaaa tttttcggaa
     5341 aaaaaattgc aaaatttttg gcatttctat aattcggcaa gtatcgaaca aattttgaaa
     5401 tttccagaaa aaactaattt ctactgttta gtgagttagt gattttttaa ctgaataaaa
     5461 attatagaga aattgatatt ttagtctgga aattggatat gttttcggca tttcgataat
     5521 cgctaggtaa cgattttttc aagttccaca atcggcattt tcgctaattg ccgaaaattc
     5581 ggattgccga acgtcaattg ccgtccaacc ctgatatgaa ctcaaaattg ttatgtttac
     5641 cgcttctaga tattttcccc agcaccatcc atttttgctc gataaataac aacttgtcca
     5701 aaataattag gtaggcggag ggaattattc aatgggtact tctaggatgt acattttccc
     5761 tctatcttgt gttccaatta aaacttagaa ctcacgaact acatcactat tgttttccgc
     5821 tagttcacat attcagagta ataactcaaa aaatgcgtga gctaggcgac caggctctac
     5881 gtactcgagc acaatgagca aacacaagca cacagaggga taagactcgg ctatatgtct
     5941 cacgggagac gtgagaacca gaaggaggcg tccgtttttt ttttcacccc ccgtccgttt
     6001 ttttttctga aaatatagac agaggagtta tagttatacg aaccaccacc accacccacc
     6061 cgcctaatat taattgtaca gaactagtat tattatactg aaaattattc aaatgaatgt
     6121 cagcttgttg aacttattct caagcttgca aaagaatgag gtgagattct atacattttg
     6181 gttggaacgt tttagtgaaa gatgttgcaa aaaaaaaagc ataaacatga ttctaattgg
     6241 ctgcaaatat cggaaaagtg attttttttt tagttaattg ttttagttaa ttgacatctt
     6301 caaaataaat tgatttataa tctttcaaaa tagagcaaag tgcggtgcaa cgctaatata
     6361 aaaaaaacag ggtaactaga gaatagaaat tagtatctag aaccagctga gactatcagg
     6421 tacaggtttc taatagttta gtcaatatat gttgattctg taatcagatg tggttattat
     6481 atgctgagta tggttctttt ggtttgaaaa attagattgc catattttta tcggttttca
     6541 gtttctgaac atttttctca tctatattaa actacaactt ctaaaatgta ggcattcaat
     6601 tggctcaaat acagctgaaa cattattaag ttcacatttc agtcttttcg ttcttataaa
     6661 acctcagttc gaagagtgtt gtttaaaaac cagatttata aaatatataa atttcattac
     6721 ggattctgac agagatattt gaatatgggg gcaagttacg tagtaattca gatgatttga
     6781 agcgcgtaat gtcttcccac gtggtgtttg tacttaatgt gagtttagct ggaaatatga
     6841 tttgtttttt acgaattggt tgattgaaga aaagatgttt tatttgttct cttagcaaga
     6901 aacagatttt catttagtac gagcgtttat ttcaaagatc tatatatctg caaaaaacaa
     6961 taataatcga atagtctgtg gcttacaagc tgttctgact cagtgcttta atagtcctgt
     7021 gaaagtgaaa aactgaaaat ttttgggtaa ttctaattgt ttttgaaagt aaagtatgca
     7081 tcattagtgg agatttttta aaaacttcaa atatctttaa cactcgatta aatattttta
     7141 gattttgaaa atgaaaatga aaattgaaaa cagttttgga aattgtgaaa ttttcgaaaa
     7201 attgagcttt gagcaatttc cagcaaaacc aattttaggt aagataagta aaaaagtaaa
     7261 tttgaagctt tgctcacgtt ttgggtatca gcatatacta gctagtccac agatatttca
     7321 aatctttttt aattcaagta acatgttttc aagattttta aagactgtgt tgcgacactg
     7381 gggaacctat ttattcaatt cagattttta aaactgtaac aactgctcca tctttgagat
     7441 ttaggaaata tttgaaatcg taaagtgaat aaattttaga ctaaaatatt tgtttcccgc
     7501 ataaaactaa aatatcctgt tattactgtt tcttcccgaa aacacctgta tccaccgaat
     7561 agtacaagca tgtcgcgtgg tagctttcat tctccttgtc ccacttctca cttctctctc
     7621 caaatttctt cttttttttt ccaaaataaa aattcaaatt ccgttgtctt ttcatttcgg
     7681 caccggtacg cacacaccgg tatcacgtca ttcaagaaaa agggaaaagg tggaaaaagt
     7741 gcattttctg cgtctattgt gattggagta ctatctaggg cacgatcggg aagaaacaag
     7801 ggagtagaag gaaattgaaa agatttggga aaaaagaagt gtgaaactgt tgtactgtac
     7861 aaacagaaca ttttgaattt taaacatcag aagctggaaa agtttcagag atttggtagt
     7921 ctttttattg gagcttggaa gttaacattt catgatttac tggccaaaac gtcccctgat
     7981 ttaaaatatt tcgagtaaat tgatgaatta ttgaaaatct ttttggagat atttgtagct
     8041 cggttttgaa cttgtaaata tttttaaaaa atttccgtaa cgggtagtca tattgaaaat
     8101 atgataaaat gttttggaag ctgagctttt ttgagatcgc atcaatataa gttcctggct
     8161 ctggtaaatc tattgagctc ggtgtcagtc taatcaagat cgatgtcagc agaagctggc
     8221 tgtacagccc ttgattggac tgagtttcag aaagctcaaa tgttgttttc aaaatgcaac
     8281 ctagcttcag atcataacga aagtatagca tgtttcatag aaattggaaa tactttcttg
     8341 aaacaagtaa atgattattt tgcacccaat gtttgatgac tacctcatgt ctttcattag
     8401 tatggcatgc aatttcaaaa tttaaattat aatatttcct ctatttgtcc tctactactc
     8461 tactgcgcca ttttaaaaaa gtaacagctc ggtccattgt gaagatatta gtgaataagt
     8521 aaattcaagc gacagacaaa taatttttga aaaatgttgt cagttggcaa tttctcaata
     8581 gaacaaatga caactgatat aagctgtaaa cgtttagatt ttattttatt tgagctaggc
     8641 ccctctcatc caaaaaggtt attaaaagtt tattgcctgt taaatttata gacttagaca
     8701 tccatccaaa tatttaaatg ttgctgttgc ttttgggtca acaaacgaca catacctgtt
     8761 agtgtcatcc ttcgttccgc gtgcacgcat cgcactgaat catcggtatt catcacctct
     8821 tctctgcaat caacgagcca aatgccaatt gggttatttg agttagaaac tcgggaagct
     8881 tcacagaaga aataattagc tgaattggca aagagagcac ctgctccccg gaatgtcata
     8941 cttttgtaga ataaaagaag ggaagacgac gacgtctccg gttcttctat cccaaacaat
     9001 accatttttt gtttcccatc tcgtcttcga tgttgctgac tcaccatcaa tatctaaaca
     9061 gaaagcaccc aatggttcgc cttttttgtc tcctttctcg acagtttctg tttttttttg
     9121 tatgttacat aaataccgta cacccaccat ctagtcaatt gtcacatatt cgaaatcaaa
     9181 agttatcaaa aactctcgtg cactttaata cacagttccg tcggccaccc cgtcaccagc
     9241 cggcgttttc ttggtgatat cattggcggt ggctctttga aaattcaaat ttggtgtcgt
     9301 cctcccgaag gtgtgggcgg agcttgcgca ccgttcgcgg tggccgttgc tctcgacagt
     9361 tttctctttc cctcgctcct ttcttctctc aaccaaacac ctgcccacac acgcaactta
     9421 cttttgtcat cgctggtccc ctgtccaaag atacaatcaa cccgtgttgt gctaatcatc
     9481 acctagaaga gtcatcttcg tgaatcagaa tcccttttct tccccctact ttttccactt
     9541 ctattcccgg ttactcacct tctgtgtttg gccccgatcg tgtgtcagca cctctgctat
     9601 ataagagagt ggagtaaagc ttatcgatac cgtcgacctc gagggggcgg taccggtaga
     9661 aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     9721 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     9781 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag
     9841 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac
     9901 tactttctgt tatggtgttc aatgcttctc gagataccca gatcatatga aacggcatga
     9961 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
    10021 tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt
    10081 taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
    10141 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
    10201 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag
    10261 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag
    10321 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
    10381 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
    10441 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
    10501 gattacacat ggcatggatg aactatacaa atgcccgggg gatcggtgga gctccaccgg
    10561 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat
    10621 gatggagatg ctggtagatc agggaactga tgcatcgtca tccgcctcca cgtccacctc
    10681 atctgtttcg agattcggag cggacacgtt catgaataca ccggatgatg tgatgatgaa
    10741 tgatgatatg gaaccgattc ctcgtgatcg gtgcaatacg tggccaatgc gtaggccgca
    10801 actcgaacca ccactcaact cgagtcccat tattcatgaa caaattcctg aagaagatgc
    10861 tgacctatac gggagcaatg agcaatgtgg acagctcggc ggagcatctt caaacgggtc
    10921 gacagcaatg cttcatactc cagatggaag caattctcat cagacatcgt ttccttcgga
    10981 tttcagaatg tccgaatcgc cagacgatac cgtatcggga aaaaagacaa cgaccagacg
    11041 gaacgcttgg ggaaatatgt catatgctga acttatcact acagccatta tggctagtcc
    11101 agagaaacgg ttaactcttg cacaagttta cgaatggatg gtccagaatg ttccatactt
    11161 cagggataag ggagattcga acagttcagc tggatggaag aactcgatcc gtcacaatct
    11221 gtctcttcat tctcgtttca tgcgaattca gaatgaagga gccggaaaga gctcgtggtg
    11281 ggttattaat ccagatgcaa agccaggaag gaatccacgg cgtacacgtg aacgatccaa
    11341 tactattgag acgactacaa aggctcaact cgaaaaatct cgccgcggag ccaagaagag
    11401 gataaaggag agagcattga tgggctccct tcactcgaca cttaatggaa attcgattgc
    11461 cggatcgatt caaacgattt ctcacgattt gtatgatgat gattcaatgc aaggagcatt
    11521 tgataacgtt ccatcatctt tccgtccccg aactcaatcg aacctctcga ttcctggatc
    11581 gtcgtctcgt gtttctccag ctattggaag tgatatctat gatgatctag aattcccatc
    11641 atgggttggc gaatcggttc cagcaattcc aagtgatatt gttgatagaa ctgatcaaat
    11701 gcgtatcgat gcaactactc atattggtgg agttcagatt aagcaggagt cgaagccgat
    11761 taagacggaa ccaattgctc caccaccatc ataccacgag ttgaacagtg tccgtggatc
    11821 gtgtgctcag aatccacttc ttcgaaatcc aattgtgcca agcactaact tcaagccaat
    11881 gccactaccg ggtgcctatg gaaactatca aaatggtgga ataactccaa tcaattggct
    11941 atcaacatcc aactcatctc cactgcctgg aattcaatcg tgtggaattg tagctgcaca
    12001 gcatactgtc gcttcttcat cggctcttcc aattgatttg gaaaatctga cacttcccga
    12061 tcagccactg atggatacta tggatgttga tgcattgatc agacatgagc tgagtcaagc
    12121 tggagggcag catattcatt ttgatttgta aattctcttc attttgtttc ccctggtgtt
    12181 gttcgaaaga gagatagcaa agcagcgagg agtgagaaat cttccgtctt catcttttca
    12241 aatccctacc tacacacact caacgatcat cacagccaga ccatcaatat tcttccaaat
    12301 tttgacgtcg ttaatttttt ttcagttttt tcaaaaactc tattttctat tttctgtcgt
    12361 ttgttcccct ttctctcgtc taattccaac acattcatcc cagtgacgtc gtgtaataat
    12421 aatataaaat acctcttctc tctttcttcc cctaatgcga aatatcgaaa aaccgttgat
    12481 tattacctct tttttcttgt tttttttttc tctctctctc tcccgtcatc caggttcttc
    12541 actctttaaa tgctacctct atcccatctt tttcgctgta aatttgtttc gcaatcaaaa
    12601 ctgctaaaac acattcccca atctgtcttt tttaattgaa tttttcaaaa aatttgattt
    12661 cttgatttct cttgtaattc tttaattttc ctcttttttt tccccctggt agcaaatgtc
    12721 tagcgattct ctttcttttt ttgtttaact ttcacatctg gccgattcga atcctccgta
    12781 tacacacaca catagtaatc tacctccaaa attttactga aagatgtgat cccctctctg
    12841 tctccctcta caaaacatta tttgtctgtt tgtgtatatt gccaccacgt cgattttaaa
    12901 ttgggggggc ccggtaccca attcgcccta tagtgagtcg tattacaatt cactggccgt
    12961 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    13021 acatcccccc ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    13081 acagttgcgt agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
    13141 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    13201 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    13261 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    13321 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    13381 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    13441 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    13501 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
    13561 aattt
//
