LOCUS       sequence-128271-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing 
            suppressor P19 as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pP19 (GB0038)
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(675..693)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(676..693)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(711..727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(711..727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(724..746)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    735..751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(759..765,766..783,784..789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    804..825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgaatggaac
       61 gagctataca aggaaacgac gctagggaac aagctaacag tgaacgttgg gatggaggat
      121 caggaggtac cacttctccc ttcaaacttc ctgacgaaag tccgagttgg actgagtggc
      181 ggctacataa cgatgagact aattcgaatc aagataatcc ccttggtttc aaggaaagct
      241 ggggtttcgg gaaagttgta tttaagagat atctcagata cgacaggacg gaagcttcac
      301 tgcacagagt ccttggatct tggacgggag attcggttaa ctatgcagca tctcgatttt
      361 tcggtttcga ccagatcgga tgtacctata gtattcggtt tcgaggagtt agtatcaccg
      421 tttctggagg ctctcgaact cttcagcatc tctgtgagat ggcaattcgg tctaagcaag
      481 aactgctaca gcttgcccca atcgaagtgg aaagtaatgt atcaagagga tgccctgaag
      541 gtactgaaac cttcgaaaaa gaaagcgagt gagcttcgag accactcatc gcccaatcac
      601 tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg
      661 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
      721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      841 ctgcccgc
//
