LOCUS       sequence-128285-        9184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg9 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD209
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2209..2922
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     CDS             2959..3675
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2959..3672
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      3696..3883
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3751..3775)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        3958..4301
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      5120..5317
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6996..7016)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6996..7014)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7035..7051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7035..7051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7048..7070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7059..7075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7083..7089,7090..7107,7108..7113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7128..7149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7266..7283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7437..8025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7517..7536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8196..8987,8988..9056))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8819..8838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9057..9161)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggttat gcgtcatggg ctttgtgttc aacgagtccg gatttattaa acaaagtatt
      721 ttaaaacctt ctcaaagaga attcacgaga gaagaattac aaaaaagatt catgctggca
      781 ggatttctta atattatatt ggcaccattt ctggtcacat actttgtttt gctttatttt
      841 ttcagatatt ttaatgagta taaaacttct cctggatcta ttggtgctcg tcaatacaca
      901 ccaattgctg aatggaaatt ccgtgagtac aatgaacttt accatatttt taagaaaaga
      961 ataagtttga gcacaacctt agctaacaag tatgttgatc agttcccgaa ggaaaagacc
     1021 aatttgtttc tgaaatttgt ttcctttatt tgtggatcat ttgtggccat tttagctttt
     1081 ctcactgtat tcgatccaga aaacttttta aactttgaaa tcacctcgga cagatctgtc
     1141 attttctaca ttactattct gggtgctata tggtctgtaa gtagaaatac aataacccag
     1201 gagtaccatg ttttcgaccc cgaagagacg ctcaaggagt tgtatgaata tacacactat
     1261 ctaccaaagg aatgggaagg aagatatcac aaagaggaaa tcaaactaga gttctgtaaa
     1321 ttgtacaact tgagaatagt aatacttttg agggaactca ctagcctgat gataacaccg
     1381 tttgtacttt ggttttcgtt gccctcgtca gccggcagga ttgtagattt cttcagagag
     1441 aattctgaat atgtggacgg attaggttac gtttgcaagt atgctatgtt taacatgaaa
     1501 aacatagacg gtgaagatac acacagcatg gatgaagaca gtttaacgaa aaagattgcc
     1561 gtgaacggaa gtcatacact caacagtaaa cgaaggagta aatttaccgc cgaagaccat
     1621 agcgataaag atttggcaaa taataaaatg ttacaatcat atgtatattt tatggatgat
     1681 tattccaaca gtgaaaactt aacagggaag taccaattgc ctgcaaaaaa aggctatccg
     1741 aataatgaag gcgattcatt tctcaacaat aagtattctt ggagaaagca atttcagcca
     1801 ggtcaaaagc cggagctatt tagaataggt aagcatgctc tcggccctgg ccataatatt
     1861 tcccctgcta tatattctac aaggaatccc ggcaagaact gggataacaa taataatggt
     1921 gatgacatta agaacgggac caataatgct acagctaaaa atgatgacaa taatggcaat
     1981 aatgatcatg aatacgtact aacagagtct ttccttgatt cgggcgcatt ccctaatcat
     2041 gatgtaatag accataataa aatgctaaac tcaaattaca atggtaatgg catactcaat
     2101 aagggtggtg tcttaggact tgttaaagag tattacaaga agtctgacgt cggaagagct
     2161 tcgtacgctg caggtcgacg gatcggtgac ggtgctggtt taattaacat gtctaaaggt
     2221 gaagaattat tcactggtgt tgtcccaatt ttggttgaat tagatggtga tgttaatggt
     2281 cacaaatttt ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa attgacctta
     2341 aaatttattt gtactactgg taaattgcca gttccatggc caaccttagt cactacttta
     2401 acttatggtg ttcaatgttt ttctagatac ccagatcata tgaaacaaca tgactttttc
     2461 aagtctgcca tgccagaagg ttatgttcaa gaaagaacta tttttttcaa agatgacggt
     2521 aactacaaga ccagagctga agtcaagttt gaaggtgata ccttagttaa tagaatcgaa
     2581 ttaaaaggta ttgattttaa agaagatggt aacattttag gtcacaaatt ggaatacaac
     2641 tataactctc acaatgttta catcatggct gacaaacaaa agaatggtat caaagttaac
     2701 ttcaaaatta gacacaacat tgaagatggt tctgttcaat tagctgacca ttatcaacaa
     2761 aatactccaa ttggtgatgg tccagtcttg ttaccagaca accattactt atccactcaa
     2821 tctgccttat ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt agaatttgtt
     2881 actgctgctg gtattaccca tggtattgat gaattgtaca aaattccagt tccaacgcgt
     2941 gatctagaga agccattcat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt
     3001 ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa
     3061 ggtgatgcta cttacggtaa attgacctta aaatttattt gtactactgg taaattgcca
     3121 gttccatggc caaccttagt cactacttta acttatggtg ttcaatgttt ttctagatac
     3181 ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa
     3241 gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt
     3301 gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt
     3361 aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta catcatggct
     3421 gacaaacaaa agaatggtat caaagttaac ttcaaaatta gacacaacat tgaagatggt
     3481 tctgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg
     3541 ttaccagaca accattactt atccactcaa tctgccttat ccaaagatcc aaacgaaaag
     3601 agagaccaca tggtcttgtt agaatttgtt actgctgctg gtattaccca tggtatggat
     3661 gaattatata aataaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
     3721 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     3781 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     3841 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
     3901 acagggtaat atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     3961 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
     4021 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
     4081 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
     4141 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
     4201 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     4261 ctaggataca gttctcacat cacatccgaa cataaacaac catgacagtc aacactaaga
     4321 cctatagtga gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa
     4381 tggaactgaa gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggaattcc
     4441 ttgaattaat tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa
     4501 tcaatgattt ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc
     4561 aatttatgat ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat
     4621 atattggtgg agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca
     4681 ctgggaatgg agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag
     4741 agccaagagg gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag
     4801 aatattctca aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta
     4861 ttgcccaacg tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg
     4921 gagttggatt agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag
     4981 ttgttagcac tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag
     5041 atccagatat tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga
     5101 ctggccaatt ataatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     5161 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     5221 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     5281 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     5341 ccatccagtg tcgaaaacga gctcgaattc atcgatgata tcagatccac tagtggccta
     5401 tgctaaggca gactgtgtcg tgtacatcat ccaatataac tatatatata actgtttcct
     5461 ttctttttcg tatttaccta aatgcatata ttattcagtt cccactaatt ttgtactaca
     5521 ataataagag aatcccttcc ctgttttaga aatgagtagt tcttttcttt ttcataaata
     5581 atacagttgt ctctgagaag cctaataaga aaaaaagagg ttaataaatt attattattg
     5641 gttatttctt tccgccacgt agttttcttt ctctttcata cttgtttttt tccgcacctt
     5701 cttcggtatt aatagtgtta acaatatgag ccatctttgc atggtagtcg ctgctctcct
     5761 tgttcttttc ttcaattctt tgtctagctt caaatttggc gtctttacgg atttctttca
     5821 tagtgaattt acgttccttc tttagttggg ccttcatctt atttatctca cttcttgttc
     5881 tatcaggatc gtacgatttt ttatcaggat tgaagttttc ttcgtattta ggagcatgcg
     5941 taggtatgga tacgggtttg tggttctgta aagccaacgg aatatgttct gtgaatttcg
     6001 ttaatttttc aactttgttc aaaatatttc ttggcttttc aaaatcagaa tactttgagg
     6061 tgtatgcact taataactgc tgaatcggta aaataatttc attgaaagca ggtaagctct
     6121 tccacacagt agagatagtt gcatctaaag attccataac atttagtaaa acggagacac
     6181 attgatcaac cggatgtgcc tcggtatcca ttgttgacaa ggtatgtaat ggaataatag
     6241 ttgatctctt ttttgtaaag tcaacgtcca atggaagccc caactcgtaa gagtctagtc
     6301 taatattttc aaaatctaat ggtttttctt gattttcttt ctcaactata aaagtgagta
     6361 atattttttg aaagaagtaa accacctccg gtatgtaccg tttggaaatg cgttgatatt
     6421 gtgaaacaat ccttaccaag acagcgccaa aagctatcct ctttaaagaa ttaaacttga
     6481 tttgttccag gaactggctc attaaaatca atgctggagt tattactaaa tggtactggt
     6541 ccgaggtgga gaaaagaata ccaataatgg aaaaaaatac cagatcacca tttgatagag
     6601 catcaaaatg gttcttttta tagcgtgctt gcatttcatt gatataatct ctacactcct
     6661 cggaaagctc tctattatat ttctctgaaa gggattttag aattgagatc aatgcatttt
     6721 gtgtacgttt aaaactttga acgtttttaa ggtagttttg attgcttaga aaaatgatgt
     6781 gtctcagtaa aacagcggtg aattttccta acttttcctt attaccttca gccaattttg
     6841 gttggtaagc tttgataata ttcttaacaa tcttaggatg atcatctaag tctaattttt
     6901 ttacttgatc taataacgca tcatgagttc tagggcatga aatggaaatg ttctttttct
     6961 ttctcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc
     7021 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7861 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8101 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8161 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8221 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8281 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8341 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8401 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8461 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8521 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8581 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8641 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8701 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8761 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8821 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8881 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8941 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9001 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9121 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9181 ccac
//
