LOCUS       sequence-128303-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for (human codon optimized) Cas9 plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB2alpha2 35s:hCas9:tNos (GB0639)
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    474..496
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact tattatttcc
       61 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
      121 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
      181 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
      241 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
      301 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
      361 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
      421 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
      481 atatattgtg gtgtaacaag cttcgtctca gtcaggagac tagagccaag ctgatctcct
      541 ttgccccgga gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg
      601 acggagaagg tgacgatacc atgttcacca ccgataatga gaagatta
//
