LOCUS       sequence-128309-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  'Donor only' control plasmid for gene targeting of tomato ANT1 gene.
            Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' 
            homology arm as a Gemini Viral Replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC150
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggagagaaca cgggggactc ttgacagtag tataatatat tatcaaatta ttatgaacag
       61 tacatctatg tcttcattgg gagtgagaaa aggttcatgg actgatgaag aagattttct
      121 tctaagaaaa tgtattgata agtatggtga aggaaaatgg catcttgttc ccataagagc
      181 tggtaactat taaattaact atcacgttat ttttatttgt ctttctgtct cattttattt
      241 gacgttatta cgaatatcat ctgaaaatgt acgtgcaggt ctgaatagat gtcggaaaag
      301 ttgtagattg aggtggctga attatctaag gccacatatc aagagaggtg actttgaaca
      361 agatgaagtg gatctcattt tgaggcttca taagctctta ggcaacaggc atgcaagttt
      421 atgttttgac aaaatttgat tagtatatat tatatatacg tgtgactatt tcatctaaat
      481 gttacgttat tttacgtaga tggtcactta ttgctggtag acttcccgga aggacagcta
      541 acgatgtgaa aaactattgg aacactaatc ttctaaggaa gttaaatact actaaaattg
      601 ttcctcgcga aaagattaac aataagtgtg gagaaattag tactaagatt gaaattataa
      661 aacctcaacg acgcaagtat ttctcaagca caatgaagaa tgttacaaac aataatgtaa
      721 ttttggacga ggaggaacat tgcatttaaa tgagtgtact tcaagtcagt gggaaatcaa
      781 taaaatgatt attttatgaa tatatttcat tgtgcaagta gatagaaatt
//
