LOCUS       sequence-128312-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C9orf114 with FLAG-tag epitope..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-C9orf114-flag
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             125..136
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcctctagac tgccggatct agctagttaa ttaaggcacc atggcggagc gcggcaggaa
       61 gcggccgtgc ggcccgggtg aacacggcca aaggattgag tggcgaaaat ggaagcaaca
      121 gaagaaagag gagaaaaaaa aatggaagga tctcaagctg atgaaaaaac tggagcggca
      181 gcgggcacag gaggaacagg caaagcgcct ggaagaggag gaggcagcgg cagagaagga
      241 ggaccgcggg cggccctaca cactgagcgt agccctgccg ggctccatcc tggacaatgc
      301 tcagtcgccg gagcttcgca cctacttggc cggtcagatt gccagagcct gtgccatctt
      361 ctgtgtggat gagatcgtgg tgtttgatga ggagggccag gatgccaaga ctgtggaggg
      421 ggaattcaca ggagttggga agaaggggca ggcgtgcgta cagctggccc ggatcctgca
      481 gtacctggag tgtccacagt acctgaggaa ggcgttcttc cccaagcacc aggatctaca
      541 gtttgcaggg ctcctgaacc ccctggacag cccccaccac atgcgtcagg atgaggaatc
      601 cgagttccga gagggcatcg tggtggatcg gcccacccgg ccaggccacg gctcctttgt
      661 caactgtggc atgaaaaagg aggtgaagat tgacaagaac ctggagcccg ggcttcgggt
      721 gactgtgcga ctgaaccagc agcagcaccc agactgcaag acctaccatg gcaaagtggt
      781 atcatcgcag gaccctcgca ccaaagctgg tctctactgg ggctacaccg tccgactggc
      841 ttcctgcctc agtgctgtgt ttgctgaggc ccccttccaa gatgggtatg acctgaccat
      901 cgggacgtca gagcgcggct cagatgtggc ctctgcccag cttcccaact tcaggcatgc
      961 tcttgtggtg ttcgggggcc tccagggtct ggaagctgga gcggatgctg acccc
//
