LOCUS       sequence-128316-         238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  'Donor only' control plasmid for gene targeting of tomato ANT1 gene.
            Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' 
            homology arm as a Gemini Viral Replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF
  TITLE     High-frequency, precise modification of the tomato genome.
  JOURNAL   Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
  PUBMED    26541286
REFERENCE   2  (bases 1 to 238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-6"; volume: "16(1):232. doi"; pages: " 
            10.1186/s13059-015-0796-9"
COMMENT     Sequence Label: pTC150
FEATURES             Location/Qualifiers
     source          1..238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    19..43
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
       61 ctatgagggt cgtacgaata attcgtatcc aacggaaata cctgatacaa tatacgctcc
      121 atcaaatacc atcacatcgt atatgctttt atagtgtgaa cacctttaac cctagtgggc
      181 gggaaatttt ctactttaaa tctggaccgc tcgtgctaaa gcactcgcga taaggggg
//
