LOCUS       sequence-128317-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved scaffold and puro/BFP
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        118..366
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     118..138
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     289..308
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(528..551)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
       61 cagcagagat ccagtttggt tagtaccggg ccctacgcgt ccaaggtcgg gcaggaagag
      121 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      181 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      241 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      301 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      361 aacaccgggt cttcgagaag acacgtttaa gagctatgct ggaaacagca tagcaagttt
      421 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgga
      481 tccaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
      541 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
      601 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctac
//
