LOCUS       sequence-128336-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for Hygromycin resistance gene plant expression
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..42
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        141..320
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 acacatacag cgacttagtt tacccgccaa tatatcctgt caggatccgt ctcatgacgg
       61 agatacatga gaattaaggg agtcacggta tgacccccgc cgatgacgcg ggacaagccg
      121 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactgagcc gcgggtttct
      181 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct
      241 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa
      301 ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga ggcaatgaaa
      361 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtg
      421 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
      481 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
      541 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
      601 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
      661 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
      721 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
      781 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
      841 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
//
