LOCUS       sequence-128337-        4417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for Hygromycin resistance gene plant expression
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)
FEATURES             Location/Qualifiers
     source          1..4417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     172..190
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(225..477)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(1558..1737)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1836..1860
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1951..2539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2031..2050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2710..3525)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3433..3452
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3517..3536
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3816..4251
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    4378..4400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 agcgcgagtc ggtcccatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
       61 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
      121 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
      181 atcacgaggc cctttcgtct tcaagaatta gcttggcaat tcccgatcta gtaacataga
      241 tgacaccghg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
      301 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
      361 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcgtcgc
      421 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
      481 atcgaagatc cattccaagc tcattccttt gccctcggac gagtgctggg gcgtcggttt
      541 ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg
      601 atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc
      661 gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca
      721 atgcggagca tatacgcccg gagccttggc gatcctgcaa gctccggatg cctccgctcg
      781 aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg
      841 acctcgtatt gggaatcccc gaacattgcc tcgctccagt caatgaccgc tgttatgcgg
      901 ccattgtccg tcaggacatt gttggagccg aatccgcgtg cacgagtgcc ggacttcggg
      961 gcagtcctcg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     1021 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     1081 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     1141 ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc
     1201 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
     1261 gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     1321 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     1381 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     1441 agctgcatca ggtcggacac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     1501 gcggtgagtt caggcttttt cattgcctcg atcgagttga gagtgaatat gagactctaa
     1561 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     1621 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     1681 ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt gcggttctgt
     1741 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac cgtgactccc
     1801 ttaattctca tgtatctccg tcatgagacg gatcctgaca ggatatattg gcgggtaaac
     1861 taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag
     1921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2401 tggtatctgc gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc
     2461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg
     2701 gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     2761 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     2821 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     2881 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     2941 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     3001 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     3061 tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac
     3121 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     3181 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
     3241 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     3301 cagccagttt agtctgacca tctcatctgt aacaacattg gcaacgctac ctttgccatg
     3361 tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga
     3421 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     3481 taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     3541 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     3601 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
     3661 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
     3721 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
     3781 tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg ccgtcgagcg
     3841 ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga aaccgctaat
     3901 gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta tccacgtgaa
     3961 atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct aataaggtca
     4021 cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca gatcaacagc
     4081 ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agtatgttca attagctttt
     4141 caattatgaa tatatatatc aattattggt cgcccttggc ttgtggacaa tgcgctacgc
     4201 gcaccggctc cgcccgtgga caaccgcaag cggttgccca ccgtcgagcg cctttgccca
     4261 caacccggcg gccggccgca acagatcgtt ttataaattt ttttttttga aaaagaaaaa
     4321 gcccgaaagg cggcaacctc tcgggcttct ggatttccga tccccggaat tagatcttgg
     4381 caggatatat tgtggtgtaa cggatccgtc tcaggag
//
