LOCUS       sequence-12834-e        9862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Rodriguez AJ, Shenoy SM, Singer RH, Condeelis J
  TITLE     Visualization of mRNA translation in living cells.
  JOURNAL   J Cell Biol. 2006 Oct 9. 175(1):67-76.
  PUBMED    17030983
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Oct 9. 175(1):67-76."
COMMENT     Sequence Label: eTC GFP beta-actin full length
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2888..2905
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             join(2915..2917,2918..2920,2921..3631)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2915..2917,2918..2920,2921..3631)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2960..2981)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3221..3240)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3568..3589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5414..5432)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5415..5432)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5452..5469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5458..5682
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5728..6156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5815..5834)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6025..6046
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6165..6185)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6170..6500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6351..6486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6413..6432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6567..7361
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6621..6640)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7231..7250
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7535..7656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7572..7591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7626..7645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7705..7721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7705..7721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7718..7740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7729..7745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7753..7759,7760..7777,7778..7783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7798..7819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7936..7953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8107..8695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8187..8206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8866..9657,9658..9726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9489..9508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9727..9831)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcttgg ccagctgaat ggaggcccag cggcaacaca ggtcctgcct
       61 ggggatcagg tctgctctgc accccacctt gctgcctgga gccgcccacc tgacaacctc
      121 tcatccctgc tctgtagatc cggtcccatc cccactgccc accccacccc cccagcactc
      181 cacccagttc aacgttccac gaacccccag aaccagccct catcaacagg cagcaagaag
      241 ggccccccgc ccatcgcccc acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg
      301 aggcagctga aagatacaag gccagggaca ggacagtccc atccccagga ggcagggagt
      361 atacaggctg gggaagtttg cccttgcgtg gggtggtgat ggaggaggct cagcaagtct
      421 tctggactgt gaacctgtgt ctgccactgt gtgctgggtg gtggtcatct ttcccaccag
      481 gctgtggcct ctgcaacctt caagggagga gcaggtccca ttggctgagc acagccttgt
      541 acgtgaactg aacaagcagc ctccttcctg gccacaggtt ccatgtcctt atatggactc
      601 atctttgcct attgcgacac acactcaatg aacacctact acgcgctgca aagagccccg
      661 caggcctgag gtgcccccac ctcaccactc ttcctatttt tgtgtaaaaa tccagcttct
      721 tgtcaccacc tccaaggagg gggaggagga ggaaggcagg ttcctctagg ctgagccgaa
      781 tgcccctctg tggtcccacg ccactgatcg ctgcatgccc accacctggg tacacacagt
      841 ctgtgattcc cggagcagaa cggaccctgc ccacccggtc ttgtgtgcta ctcagtggac
      901 agacccaagg caagaaaggg tgacaaggac agggtcttcc caggctggct ttgagttcct
      961 agcaccgccc cgcccccaat cctctgtggc acatggagtc ttggtcccca gagtccccca
     1021 gcggcctcca gatggtctgg gagggcagtt cagctgtggc tgcgcatagc agacatacaa
     1081 cggacggtgg gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag
     1141 gtcacccact aacgccccag gcctggtctt ggctgggcgt gactgttacc ctcaaaagca
     1201 ggcagctcca gggtaaaagg tgccctgccc tgtagagccc acttccttcc cagggctgcg
     1261 gctgggtagg tttgtagcct tcatcacggg ccacctccag ccactggacc gctggcccct
     1321 gccctgtcct ggggagtgtg gtcctgcgac tctaatggcc gcaagccacc tgactccccc
     1381 aacaccacac tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta
     1441 gctagctgag ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa
     1501 gtcggcaagg gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg
     1561 agcgtacaga acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc
     1621 tcagtgccca agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga
     1681 cgggcaggcc gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg
     1741 gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg
     1801 ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa
     1861 aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc
     1921 gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg
     1981 cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccg
     2041 cgagcacaga gcctcgcctt tgccgatccg ccgcccgtcc acacccgccg ccaggtaagc
     2101 ccggccagcc gaccggggca tgcggccgcg gcccttcgcc cgtgcagagc cgccgtctgg
     2161 gccgcagcgg ggggcgcatg gggcggaacc ggaccgccgt ggggggcgcg ggagaagccc
     2221 ctgggcctcc ggagatgggg gacaccccac gccagttcgc aggcgcgagg ccgcgctcgg
     2281 gcgggcgcgc tccgggggtg ccgctctcgg ggcgggggca accggcgggg tctttgtctg
     2341 agccgggctc ttgccaatgg ggatcgcacg gtgggcgcgg cgtagccccc gtcaggcccg
     2401 gtgggggctg gggcgccatg cgcgtgcgcg ctggtccttt gggcgctaac tgcgtgcgcg
     2461 ctgggaattg gcgctaattg cgcgtgcgcg ctgggactca atggcgctaa tcgcgcgtgc
     2521 gttctggggc ccgggcgctt gcgccacttc ctgcccgagc cgctggcgcc cgagggtgtg
     2581 gccgctgcgt gcgcgcgcgc gacccggtcg ctgtttgaac cgggcggagg cggggctggc
     2641 gcccggttgg gagggggttg gggcctggct tcctgccgcg cgccgcgggg acgcctccga
     2701 ccagtgtttg ccttttatgg taataacgcg gccggcccgg cttcctttgt ccccaatctg
     2761 ggcgcgcgcc ggcgccccct ggcggcctaa ggactcggcg cgccggaagt ggccagggcg
     2821 ggggcgactt cggctcacag cgcgcccggc tattctcgca gctcaagctt ccaccatgtt
     2881 cctcaactgc tgcccaggat gttgtatgga gccaatggtg agcaagggcg aggagctgtt
     2941 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3001 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3061 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3121 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3181 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3241 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3301 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3361 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3421 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3481 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     3541 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3601 gatcactctc ggcatggacg agctgtacaa gggatccact agtggcatgg atgatgatat
     3661 cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga
     3721 tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt
     3781 gggcatgggt cagaaggatt cctatgtggg cgacgaggcc cagagcaaga gaggcatcct
     3841 caccctgaag taccccatcg agcacggcat cgtcaccaac tgggacgaca tggagaaaat
     3901 ctggcaccac accttctaca atgagctgcg tgtggctccc gaggagcacc ccgtgctgct
     3961 gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag atgacccaga tcatgtttga
     4021 gaccttcaac accccagcca tgtacgttgc tatccaggct gtgctatccc tgtacgcctc
     4081 tggccgtacc actggcatcg tgatggactc cggtgacggg gtcacccaca ctgtgcccat
     4141 ctacgagggg tatgccctcc cccatgccat cctgcgtctg gacctggctg gccgggacct
     4201 gactgactac ctcatgaaga tcctcaccga gcgcggctac agcttcacca ccacggccga
     4261 gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg acttcgagca
     4321 agagatggcc acggctgctt ccagctcctc cctggagaag agctacgagc tgcctgacgg
     4381 ccaggtcatc accattggca atgagcggtt ccgctgccct gaggcactct tccagccttc
     4441 cttcctgggc atggagtcct gtggcatcca cgaaactacc ttcaactcca tcatgaagtg
     4501 tgacgtggac atccgcaaag acctgtacgc caacacagtg ctgtctggcg gcaccaccat
     4561 gtaccctggc attgccgaca ggatgcagaa ggagatcact gccctggcac ccagcacaat
     4621 gaagatcaag atcattgctc ctcctgagcg caagtactcc gtgtggatcg gcggctccat
     4681 cctggcctcg ctgtccacct tccagcagat gtggatcagc aagcaggagt atgacgagtc
     4741 cggcccctcc atcgtccacc gcaaatgctt ctaggcggac tatgacttag ttgcgttaca
     4801 ccctttcttg acaaaaccta acttgcgcag aaaacaagat gagattggca tggctttatt
     4861 tgtttttttt gttttgtttt ggtttttttt ttttttttgg cttgactcag gatttaaaaa
     4921 ctggaacggt gaaggtgaca gcagtcggtt ggagcgagca tcccccaaag ttcacaatgt
     4981 ggccgaggac tttgattgca cattgttgtt tttttaatag tcattccaaa tatgagatgc
     5041 gttgttacag gaagtccctt gccatcctaa aagccacccc acttctctct aaggagaatg
     5101 gcccagtcct ctcccaagtc cacacagggg aggtgatagc attgctttcg tgtaaattat
     5161 gtaatgcaaa atttttttaa tcttcgcctt aatacttttt tattttgttt tattttgaat
     5221 gatgagcctt cgtgcccccc cttccccctt ttttgtcccc caacttgaga tgtatgaagg
     5281 cttttggtct ccctgggagt gggtggaggc agccagggct tacctgtaca ctgacttgag
     5341 accagttgat tacgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
     5401 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     5461 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5521 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5581 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5641 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5701 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5761 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5821 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5881 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5941 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6001 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6061 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     6121 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     6181 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca
     6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6541 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6601 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6661 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6721 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6781 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6841 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6901 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6961 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7021 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7081 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     7141 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7201 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7261 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7321 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7381 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7441 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7501 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7681 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8281 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8521 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9841 ccgaaaagtg ccacctgacg tc
//
