LOCUS       sequence-128346-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for Firefly Luciferase Protein plant expression
            driven by the Nos promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB Pnos:Luciferase:Tnos (GB0112)
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(37..55)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    243..267
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(358..946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(438..457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgggaattg ccaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
       61 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      181 gacaataacc ctgataaatg cttcaataat gggaccgact cgcgctgtca tgagacgaat
      241 tctgacagga tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt gagatctcat
      301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      841 cggaagaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      961 cttt
//
