LOCUS       sequence-12835-e        9283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Rodriguez AJ, Shenoy SM, Singer RH, Condeelis J
  TITLE     Visualization of mRNA translation in living cells.
  JOURNAL   J Cell Biol. 2006 Oct 9. 175(1):67-76.
  PUBMED    17030983
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Oct 9. 175(1):67-76."
COMMENT     Sequence Label: eTC GFP ß-actin Delta-Zip
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2888..2905
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             join(2915..2917,2918..2920,2921..3631)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2915..2917,2918..2920,2921..3631)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2960..2981)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3221..3240)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3568..3589
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4835..4853)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4836..4853)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4873..4890)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4879..5103
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5149..5577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5236..5255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5446..5467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5586..5606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5591..5921
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5772..5907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5834..5853
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5988..6782
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6042..6061)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6652..6671
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6956..7077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6993..7012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7047..7066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7126..7142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7126..7142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7139..7161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7150..7166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7174..7180,7181..7198,7199..7204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7219..7240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7357..7374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7528..8116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7608..7627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8287..9078,9079..9147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8910..8929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9148..9252)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcttgg ccagctgaat ggaggcccag cggcaacaca ggtcctgcct
       61 ggggatcagg tctgctctgc accccacctt gctgcctgga gccgcccacc tgacaacctc
      121 tcatccctgc tctgtagatc cggtcccatc cccactgccc accccacccc cccagcactc
      181 cacccagttc aacgttccac gaacccccag aaccagccct catcaacagg cagcaagaag
      241 ggccccccgc ccatcgcccc acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg
      301 aggcagctga aagatacaag gccagggaca ggacagtccc atccccagga ggcagggagt
      361 atacaggctg gggaagtttg cccttgcgtg gggtggtgat ggaggaggct cagcaagtct
      421 tctggactgt gaacctgtgt ctgccactgt gtgctgggtg gtggtcatct ttcccaccag
      481 gctgtggcct ctgcaacctt caagggagga gcaggtccca ttggctgagc acagccttgt
      541 acgtgaactg aacaagcagc ctccttcctg gccacaggtt ccatgtcctt atatggactc
      601 atctttgcct attgcgacac acactcaatg aacacctact acgcgctgca aagagccccg
      661 caggcctgag gtgcccccac ctcaccactc ttcctatttt tgtgtaaaaa tccagcttct
      721 tgtcaccacc tccaaggagg gggaggagga ggaaggcagg ttcctctagg ctgagccgaa
      781 tgcccctctg tggtcccacg ccactgatcg ctgcatgccc accacctggg tacacacagt
      841 ctgtgattcc cggagcagaa cggaccctgc ccacccggtc ttgtgtgcta ctcagtggac
      901 agacccaagg caagaaaggg tgacaaggac agggtcttcc caggctggct ttgagttcct
      961 agcaccgccc cgcccccaat cctctgtggc acatggagtc ttggtcccca gagtccccca
     1021 gcggcctcca gatggtctgg gagggcagtt cagctgtggc tgcgcatagc agacatacaa
     1081 cggacggtgg gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag
     1141 gtcacccact aacgccccag gcctggtctt ggctgggcgt gactgttacc ctcaaaagca
     1201 ggcagctcca gggtaaaagg tgccctgccc tgtagagccc acttccttcc cagggctgcg
     1261 gctgggtagg tttgtagcct tcatcacggg ccacctccag ccactggacc gctggcccct
     1321 gccctgtcct ggggagtgtg gtcctgcgac tctaatggcc gcaagccacc tgactccccc
     1381 aacaccacac tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta
     1441 gctagctgag ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa
     1501 gtcggcaagg gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg
     1561 agcgtacaga acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc
     1621 tcagtgccca agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga
     1681 cgggcaggcc gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg
     1741 gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag gcggccaacg
     1801 ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa
     1861 aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc
     1921 gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga gcggccgcgg
     1981 cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccg
     2041 cgagcacaga gcctcgcctt tgccgatccg ccgcccgtcc acacccgccg ccaggtaagc
     2101 ccggccagcc gaccggggca tgcggccgcg gcccttcgcc cgtgcagagc cgccgtctgg
     2161 gccgcagcgg ggggcgcatg gggcggaacc ggaccgccgt ggggggcgcg ggagaagccc
     2221 ctgggcctcc ggagatgggg gacaccccac gccagttcgc aggcgcgagg ccgcgctcgg
     2281 gcgggcgcgc tccgggggtg ccgctctcgg ggcgggggca accggcgggg tctttgtctg
     2341 agccgggctc ttgccaatgg ggatcgcacg gtgggcgcgg cgtagccccc gtcaggcccg
     2401 gtgggggctg gggcgccatg cgcgtgcgcg ctggtccttt gggcgctaac tgcgtgcgcg
     2461 ctgggaattg gcgctaattg cgcgtgcgcg ctgggactca atggcgctaa tcgcgcgtgc
     2521 gttctggggc ccgggcgctt gcgccacttc ctgcccgagc cgctggcgcc cgagggtgtg
     2581 gccgctgcgt gcgcgcgcgc gacccggtcg ctgtttgaac cgggcggagg cggggctggc
     2641 gcccggttgg gagggggttg gggcctggct tcctgccgcg cgccgcgggg acgcctccga
     2701 ccagtgtttg ccttttatgg taataacgcg gccggcccgg cttcctttgt ccccaatctg
     2761 ggcgcgcgcc ggcgccccct ggcggcctaa ggactcggcg cgccggaagt ggccagggcg
     2821 ggggcgactt cggctcacag cgcgcccggc tattctcgca gctcaagctt ccaccatgtt
     2881 cctcaactgc tgcccaggat gttgtatgga gccaatggtg agcaagggcg aggagctgtt
     2941 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3001 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3061 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3121 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3181 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3241 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3301 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3361 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3421 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3481 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     3541 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3601 gatcactctc ggcatggacg agctgtacaa gggatccact agtggcatgg atgatgatat
     3661 cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga
     3721 tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg gcgtgatggt
     3781 gggcatgggt cagaaggatt cctatgtggg cgacgaggcc cagagcaaga gaggcatcct
     3841 caccctgaag taccccatcg agcacggcat cgtcaccaac tgggacgaca tggagaaaat
     3901 ctggcaccac accttctaca atgagctgcg tgtggctccc gaggagcacc ccgtgctgct
     3961 gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag atgacccaga tcatgtttga
     4021 gaccttcaac accccagcca tgtacgttgc tatccaggct gtgctatccc tgtacgcctc
     4081 tggccgtacc actggcatcg tgatggactc cggtgacggg gtcacccaca ctgtgcccat
     4141 ctacgagggg tatgccctcc cccatgccat cctgcgtctg gacctggctg gccgggacct
     4201 gactgactac ctcatgaaga tcctcaccga gcgcggctac agcttcacca ccacggccga
     4261 gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg acttcgagca
     4321 agagatggcc acggctgctt ccagctcctc cctggagaag agctacgagc tgcctgacgg
     4381 ccaggtcatc accattggca atgagcggtt ccgctgccct gaggcactct tccagccttc
     4441 cttcctgggc atggagtcct gtggcatcca cgaaactacc ttcaactcca tcatgaagtg
     4501 tgacgtggac atccgcaaag acctgtacgc caacacagtg ctgtctggcg gcaccaccat
     4561 gtaccctggc attgccgaca ggatgcagaa ggagatcact gccctggcac ccagcacaat
     4621 gaagatcaag atcattgctc ctcctgagcg caagtactcc gtgtggatcg gcggctccat
     4681 cctggcctcg ctgtccacct tccagcagat gtggatcagc aagcaggagt atgacgagtc
     4741 cggcccctcc atcgtccacc gcaaatgctt ctaggaattc tgcagatatc catcacactg
     4801 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     4861 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4921 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4981 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5041 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5101 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5161 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5221 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5281 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
     5341 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5401 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5461 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
     5521 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5581 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa
     5641 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5701 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5761 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5821 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5881 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5941 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     6001 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6061 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6121 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6181 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6241 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6301 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6361 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6421 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6481 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6541 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6601 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6661 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6721 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6781 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6841 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6901 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7081 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     7741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9241 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
