LOCUS       sequence-12836-e        7726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Gandotra S, Lebron MB, Ehrt S
  TITLE     The Mycobacterium tuberculosis proteasome active site threonine is 
            essential for persistence yet dispensable for replication and 
            resistance to nitric oxide.
  JOURNAL   PLoS Pathog. 2010 Aug 12;6(8):e1001040. doi: 
            10.1371/journal.ppat.1001040
  PUBMED    20711362
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2010-08-12"; volume: "6(8):e1001040. doi"; pages: "
            10.1371/journal.ppat.100104"
COMMENT     Sequence Label: pGMCS-0X-P38-PanBmGFP
FEATURES             Location/Qualifiers
     source          1..7726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(30..52)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    596..730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(633..652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     687..706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(926..1514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1006..1025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1837..2628)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(5202..5248)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5505..5527
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5524..5540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5524..5540
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5560..5580
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5584..5608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6100..6124)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(7719..7726,1..13))
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
       61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggggattg
      241 gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct
      301 accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg
      361 tatttcgcgt aaggaaatcc attatgtact atttaaaaaa cacaaacttt tggatgttcg
      421 gtttattctt tttcttttac ttttttatca tgggagccta cttcccgttt ttcccgattt
      481 ggctacatga catcaaccat atcagcaaaa gtgatacggg tattattttt gccgctattt
      541 ctctgttctc gctattattc caaccgctgt ttggtctgct ttctgacaaa ctcggaactt
      601 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      661 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      721 tgtctggatc ctctagagtc gacctgcagg tttaaacgcg gccgcacgcg ttgcgctcgg
      781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaact
     1561 agcctaaagg tcatccaccg gatcagctta gtaaagccct cgctagattt taatgcggat
     1621 gttgcgatta cttcgccaac tattgcgata acaagaaaaa gccagccttt catgatatat
     1681 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     1741 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc
     1801 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
     1861 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1921 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1981 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     2041 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     2101 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     2161 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     2221 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     2281 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     2341 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     2401 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     2461 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2521 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2581 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga tgtttaactt
     2641 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2701 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2761 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2821 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2881 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2941 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3001 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3061 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3121 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3181 gttcgcccag cttctgtatg gaacgggcat gcggatcagt gagggtttgc aactgcgggt
     3241 caaggatctg gatttcgatc acggcacgat catcgtgcgg gagggcaagg gctccaagga
     3301 tcgggccttg atgttacccg agagcttggc acccagcctg cgcgagcagg ggaattgatc
     3361 cggtggatga ccgctagctg gcgggaacgg caatgctcct ggcggcggcg gggctggtgc
     3421 ccaggtctcc gcacagagcg gcggtgctgg cgctcgcggc caggcgtggt tcttcgcgta
     3481 ctgacaagaa acccccctct ttaggactca gtgtccttgg gaggggggct ttttgcgttt
     3541 caggaggtct tggccagctt ggacatcgcc tcagcgatag cctcgtcgcg ggcctcagac
     3601 gccatctggt acttcatcgc catcctagga gtcgtgtgac cgagacgggc catcagctcc
     3661 ttggtcgtcg cacctgcctg agcggcgaac gtagcgccga cagcgcggag gtcgtggatg
     3721 cggagttccg gccgaccgat cttggcgtag ccacgcttca gcgacttggt gaacgcggac
     3781 ttcgacagcc ggttgccctg cgtcgtggtc accaggaatg cctcggggcc cttgttcatc
     3841 ttcgtacggt ccttcatgtg cgctcggatc atctccgcga cgtgaggcgg aaccgtcaca
     3901 ggacgcttcg accggacggt cttggcgttg ccaacgacga tcttgttccc cacgcgggaa
     3961 gcgccacggc gcacccggag cttcatcgtc atgccgtcgt ccacgatgtc cttgcggcga
     4021 agctcgatca gctctccgaa ccggaggctc gtccacgcca ggatgtatgc cgcgatccgg
     4081 tagtgctcga agatctcagc ggcgacgatg tccagctcct caggcgtcag cgcctctacg
     4141 tcgcgctcat cggctgcctt ctgctcgatc cggcacgggt tctctgcgat cagcttgtcc
     4201 tcgaccgctg tgttcatcac cgcccggagg acgttgtagg catgccggcg ggcagtcggg
     4261 tgcttcctac ccatcccggc ccaccacgca cgcaccagag ctggcgtcat ctctgtgacc
     4321 gccacttcac ctagcaccgg gtagatgcgg cgctccgcgt gcccgctgta cagatccctg
     4381 gtgccgtctg cgaggtcgcg ctccacgagc cacttccggg tgtactcctc cagcgtgatg
     4441 gcgctggcgg ctgccttctt cgcccggtcc tgtggagggg tccaggtctc catctcgatg
     4501 agccgcttct cgcccgcgag ccaggcttcg gcgtccatct tgttgtcgta ggtctgcagc
     4561 gcgtagtacc tcacaccgtc ctgcgggttg acgtatgagg cttggatcct cccgctgcgc
     4621 tgagtcttca gcgatcccca tccgcgacgt gccaactagg tctcctctcg tcgtgaacaa
     4681 ggctaccggg ttgcaactcc tgtgcaactc tcaggcttca acgcgcttct acgacctgca
     4741 atttctttcc acttagagga tgcagccgag agggggtaaa aacctatctt gaccggccca
     4801 tatatgtggt cggcagacac ccattcttcc aaactagcta cgcgggttcg attcccgtcg
     4861 cccgctccgc tggtcagagg gtgttttcgc cctctggcca tttttctttc caggggtctg
     4921 caactcttgt gcgactcttc tgacctgggc atacgcggtt gcaacgcatc cctgatctgg
     4981 ctactttcga tgctgacaaa cgaatagagc cccccgcctg cgcgaacaga cgaggggcat
     5041 tcacaccaga ttggagctgg tgcagtgaag agaatagacc gggacaaggt tgcaccggga
     5101 gttgcagcgg tcggaaccct cgccgtcggc gggctggcgt tcgccctgtc gttcacggct
     5161 ctagctgatc accgcggcca tgatggccgg acaaacaaca gataaaacga aaggcccagt
     5221 ctttcgactg agcctttcgt tttatttgat gcctggcagt cgatcgtacg ctagttaact
     5281 acgtcgacat cgatttaatt aatatgcatc aattgattta aatactagtg ttaactattt
     5341 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcnaag gggacctcta
     5401 gggtccccag ctggctagcg agtcatgagg tnaccatgtt ctttcctgcg ttatcccctg
     5461 attctgtgga taaccgtatt accgcctttg agtgagctga taccagcgga taacaatttc
     5521 acacaggaaa cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg
     5581 aattcaagtt tgtacaaaaa agcaggctta ttggatcacg ccgcgagcgt gtcggtgttc
     5641 gaagacctgg gaatccagca ggcgcccaac agcaccgtct gctccgtctt ctccagaaag
     5701 gcgactacgc ggtcctcctc gtcggtgggt acgcagccga atgcccgcgg gtcgcccacc
     5761 cgcaccagtt gcagcgtgac atcggctcga ttgcttcggt ggaagtccag cagttgggcc
     5821 agatccgcga gggccccccg tgaacatcgt cgtggtaacc agcgtgtcgg ctttggctgt
     5881 ggccgtggtg cattcggtgg cgtttgcgat caaggggcgt cacggcgagg acctggtcga
     5941 ccgctggaac cgtgagggca tcggtgcaca ccgcgggatc agaccgtcga cgaaccattt
     6001 gaatccgacc ggtacctcaa cgagttgacg gccgatcacg tccaggttat gttcgatgac
     6061 gatcaccgta ttgcccttgt cgaccaggcc gttgatccga cccagctttc ttgtacaaag
     6121 tggcagaaag gaggttaata atgtctgagc agactatcta tggggccaat acccccggag
     6181 gctccgggcc gcggaccaag atccgcaccc accacctaca gagatggaag gccgacggcc
     6241 acaagtgggc catgctgacg gcctacgact attcgacggc ccggatcttc gacgaggccg
     6301 gcatcccggt gctgctggtc ggtgattcgg cggccaacgt cgtgtacggc tacgacacca
     6361 ccgtgccgat ctccatcgac gagctgatcc cgctggtccg tggcgtggtg cggggtgccc
     6421 cgcacgcact ggtcgtcgcc gacctgccgt tcggcagcta cgaggcgggg cccaccgccg
     6481 cgttggccgc cgccacccgg ttcctcaagg acggcggcgc acatgcggtc aagctcgagg
     6541 gcggtgagcg ggtggccgag caaatcgcct gtctgaccgc ggcgggcatc ccggtgatgg
     6601 cacacatcgg cttcaccccg caaagcgtca acaccttggg cggcttccgg gtgcagggcc
     6661 gcggcgacgc cgccgaacaa accatcgccg acgcgatcgc cgtcgccgaa gccggagcgt
     6721 ttgccgtcgt gatggagatg gtgcccgccg agttggccac ccagatcacc ggcaagctta
     6781 ccattccgac ggtcgggatc ggcgctgggc ccaactgcga cggccaggtc ctggtatggc
     6841 aggacatggc cgggttcagc ggcgccaaga ccgcccgctt cgtcaaacgg tatgccgatg
     6901 tcggtggtga actacgccgt gctgcaatgc aatacgccca agaggtggcc ggcggggtat
     6961 tccccgctga cgaacacagt ttctcgggct cgggctcggg catgtcgaag ggcgaggagc
     7021 tgttcaccgg cgtcgtcccg atcctggtcg agctggacgg tgacgtcaac ggccacaagt
     7081 tctccgtctc cggcgagggt gagggcgacg ccacctacgg caagctgacc ctgaagttca
     7141 tctgcaccac cggtaagctg ccggtcccgt ggccgaccct ggtcaccacc ctgacctacg
     7201 gcgtccagtg cttctcccgc tacccggacc acatgaagcg ccacgacttc ttcaagtccg
     7261 ccatgccgga gggttacgtc caggagcgca ccatctcctt caaggacgac ggtaactaca
     7321 agacgcgtgc cgaggtcaag ttcgagggcg acaccctggt caaccgcatc gagctgaagg
     7381 gcatcgactt caaggaggac ggtaacatcc tgggccacaa gctggagtac aactacaact
     7441 cccacaacgt ctacatcacc gcggacaagc agaagaacgg catcaaggcc aacttcaaga
     7501 cccgccacaa catcgaggac ggtggcgtcc agctagccga ccactaccag cagaacaccc
     7561 cgatcggcga cggcccggtc ctgctgccgg acaaccacta cctgtccacc cagtccgccc
     7621 tgtccaagga cccgaacgag aagcgcgacc acatggtcct gctggagttc gtcaccgccg
     7681 ccggcatcac ccacggcatg gacgagctgt acaagtagca acttta
//
