LOCUS       sequence-128369-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Cas9 protein and puromycin 
            resistance from EFS promoter and a C9orf114 targeting synthetic 
            single-guide RNA (sgRNA) element from U6 promoter. Lentiviral 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: lentiCRISPR - C9orf114 sgRNA 2
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        225..300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    348..465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tctttgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
      181 tcttgtggaa aggacgaaac accgcaggcg ggctcacctc cgtggtttta gagctagaaa
      241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 ttttttgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
      361 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      421 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      481 gacagcagag atccactttg gcgccggctc gagtggctcc ggtgcccgtc agtgggcaga
      541 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      601 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc t
//
