LOCUS       sequence-12837-e       17290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17290)
  AUTHORS   Mapes J, Chen JT, Yu JS, Xue D
  TITLE     Somatic sex determination in Caenorhabditis elegans is modulated by 
            SUP-26 repression of tra-2 translation.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Oct 19. 107(42):18022-7.
  PUBMED    20921392
REFERENCE   2  (bases 1 to 17290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Oct 19. 107(42):18022-7."
COMMENT     Sequence Label: pJM614-TRA-2::GFP
FEATURES             Location/Qualifiers
     source          1..17290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12924..12952)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     13700..13720
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(14730..14752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14852..14871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14949..14967)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15035..15139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15140..15208,15209..16000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15358..15377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16171..16759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16660..16679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16913..16930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17047..17068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17083..17088,17089..17106,17107..17113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17121..17137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17126..17148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17145..17161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17145..17161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttaagg tgcacggccc acgtggccac tagtacttct cgagttgaga ttcgccaaca
       61 gcttaatttt tgcgtatcgg ggctgcgtgc gcgcggccct gaaaaatatt aaagtttagt
      121 gaatctgaaa atagggaagg aaattttaga ttggccagtg tgatgacgac gatggaggaa
      181 tgccaatgga gttcgttagg gaaggaacgc tcggagacct ggtcagggaa actcgtcgtg
      241 ttctgaaaat tctacccacc gacaaagtac gttgacccac tgattccaag cgtccagtta
      301 tattttttag atctacggat actatcgcat tgatgacaat aagaagtcga aactcgatgt
      361 gacaaaagat acaatccgat cttcttgttc ttctttgaac atcaacgagg ttcattttaa
      421 aacacccttg acttcattct tcattttaat atttacagaa cactactgta cttgtcgata
      481 ccacgggaat cgtgtcgaga ccaaagaaac aatagaatga gctcaattaa ttgatactca
      541 attacgattt gtcattctaa taaacaatat ttcttcaaat aaattccttt ccgatctaaa
      601 tacacatctt atcgtttctt tcctccagaa ttctgcagaa tttataatat acaacaaatc
      661 ttgctaacga cagtatacaa caaatctggc taacgacact ccgtaataac gcatgcgcgt
      721 gttgtttagc ccgtaaatat acaacagtta ttttttaaac aaaaaaacaa ttccagcaga
      781 gttgcaagct ttctcttttt tttcctaaca ttttaaacat atttttttcg ttcaaataac
      841 aatttcagat aaaaatgcac gaaatgcaag ggatactttt gtgttctgga ggtggcacac
      901 gcatgcctgt ccttacacgc catgttcaaa aatgcctact gcctgttgtc ggagtgccaa
      961 tgttccttta tccactttcc tcgcttctaa gaacaggaat tactggtaac attgattttt
     1021 tatcgttaaa tatgattgag taattaaact tttaagatat caagatattt gttcgtgaag
     1081 ttcttcaact aactctcgaa aaagaagtaa aaaagtcgaa gctattagaa aagtatccag
     1141 cacatattga atatatttgt gtcaatcaag aggattttgg aaccgcggat ctattgaaga
     1201 atcatcattc taaaattacg gtaagtcaat cagctgaaaa agaagttacc ttaataattc
     1261 taatttttac agaaagatgc attgattgtt tcctgtgatt ttatcagcga tgccagtctc
     1321 attccacttg ttgatttctt ccgagctaca aattccacgc ttgtcgctct tattgcagat
     1381 acgtgtgtca atgctccggc tccgggatca aaatcgaaaa aacctaaagg tattatttat
     1441 ttcaaccttc ctctaattta tcaaaatatt aatttacagc caccgatgta atggcaatag
     1501 tagaatcgac aggtcaactt gcattccttt gcggagatga tgattttgat gctccattgg
     1561 ttatggagaa aagtttgaaa atattcccat cgatcaaatt aacttcaaag tacaatgatt
     1621 gtcatgttta tgctatacgt cataaagttc ttcttaatct gtcaaaatca aagtaagtgt
     1681 ttctttgaat gtgttttact ctaaatgtat aatttctaga cacatttcat cattcaaagc
     1741 cgattttgtg cctcttctga ttgataaaca atttgaaccg gattctgata tcaaatgctt
     1801 tgcatatcgt cttcctcatg aaaatggatt tgtaactgca catgcgaata ctcttggctc
     1861 ttatttcgaa gtgaataaag ctgtaagtgc taataatgca ttatcatata gcctttaaat
     1921 tgttttttta gattcaaaaa tcttttactc gattgatgga atatcgcgga aatgggaaga
     1981 atttcaacta caaaactgat aaaattgctg cccacgagag tcggatcgaa gaaagcgcag
     2041 aggtaatact tgttcaaaaa tagttaggga acatttccat tcagattgac aaggattcag
     2101 ttatcaaacg gtctttcatc tcggataatt gtcggattgg tgaaaaaaca aagctaaaag
     2161 agagtatcat tgccaaaggc gttgtcattg gaaatgggtt agttttggga ttttcgtagc
     2221 gcgagaagtt tcggatgagc tttcggaaaa gatattttct taataaatag acaaacatat
     2281 gaaaactttc gatttaagaa ttcttctgtt atcacaatta ctgatttctt gaattaaaac
     2341 ttgaatttat acttttccat tccagtgcct ccataagcaa ctctattatt tgtgatggag
     2401 tagaaatcgg agagaatgct gacgtcacca attgcattgt tgctaaggat cagaaggtac
     2461 ctgctaaagg ttagttgttt tcattagtgt tgtttttttt ttaatttctc gtgtttcttt
     2521 ataaatcaac gattttcagg caaagttcaa aacgaggtag tagaagatgg agaagacgaa
     2581 gaatggaccg atgattaaca atcttggaat tttaggaata attggagcct ttttgtgttt
     2641 tctctttttt ttacgcggtt gttttatttt tgttttctga taaatttatt gaagattaag
     2701 tttttttgaa attttcgttt tcctctatct ttcactccaa tgataggttc tctaacccta
     2761 caaattattt tgagtaatat ttttatttac gatattattt tactaacaga taataatgga
     2821 acaatgaaat tgaaatacaa taaacttctc gtttcggtgg tgattgtcac ctttgtaaca
     2881 ttcgggttgc tcttagcgga atgttttgga aaatcaattg attatcaaga aaaatcaata
     2941 tttccatcgt ttgtcagcca gggattcttc gaaactcgta cgaacaatga agaatacatt
     3001 atcgaggtat aaccttttca atttggaatt cagatactga aagtttttaa aattttaaaa
     3061 tggctatttg aattagatga caaggtctaa atatttttaa ctttaagcta agtatgacat
     3121 gattttgtga acaaacaaat gtttttaaat tttcaaacta tataaatata aattttctag
     3181 aaaatcgctc aaacccagga gaatggagta gatatgcgat caacgttaca tttcactcaa
     3241 catggatact tactgaacaa catttcaaat ttgaaaatta aatttcgcca aaagtgagtt
     3301 tatttcattc gtactcttcc ataaaacgtc caacatattt tagaacatac acactcaatg
     3361 atgtctgctt taaaccacat attacaatat tccaacaatc ctcttcatcc gatcaaaacg
     3421 aatatccaca ttatatccag gttttcttga cttttagaaa attaaaattt cattaaaata
     3481 gtaatttcag aggcttcttc ttgaaatgca acgtctgtca ccatgtctta tcgtgacacc
     3541 tctaaactgt ttctatgata tctaccgtat tcatggtgaa atcagtaatt ggaataagaa
     3601 taccgacttt ttgaatcgaa gacttagaaa ttcttatatt gaagctattg gagaaaatga
     3661 tgagaggccg tatgtgaaat caaattgtaa taaatcaaaa taaaaatatg ttaaaaaaaa
     3721 aacaaataaa tatgtattta gatggaccta gcctgatcaa aagttgggcc gatcacatgt
     3781 ttgatttacc atcgaagtca ttcaccaact caactaaaga tgcattattt cagaagatta
     3841 agctttggct tttggtagga atacatttaa ttataatctt cttttttcaa caaccaacaa
     3901 aatcttcaga gcatagaacc acgccaaaaa acatgtgcag cttcaattca ttcctgtgac
     3961 acacctctgg actctgaaca ctacttcaat atatgtacag atatgcagtc tgttgataat
     4021 ttcgctgaaa agaagacaaa ggtattaata tatgcataac cacgcaatta ctgaaaaaaa
     4081 aaagaaaaat tgcagttcaa attagaagat gtcgatgaag aattcgcaat gaatctcgat
     4141 tgtgtggatg atcaagaaca gttcattgaa tggatgcagg agttggagat cagaaaaatg
     4201 tattctcatg taactgaaaa gccagattat ccaaatgttg taaatcaaac atgcgataag
     4261 atttttcatg atttgaatag cactggaatt gaatttttcg acggatcgag aagcttcagt
     4321 agtacaaaat ctcaatttga tacaatgcaa acggaaattg tcctgttaac tcctgaaatg
     4381 ctcttatcag ctatgcaaca ttcagacttt gtgaatggtt ttgaatcaat ttggactatt
     4441 gaaaaagctg aagaacttat tcatgaattc cgattagcct tgaaagaaga aactgagaag
     4501 ttcaaagaga atagaatgag caaaatgata agagttacat cacgagtttt ggataatact
     4561 gtaactacga aacttcaatc attttcggag aaacaaacaa tacattttgt ggttaatgta
     4621 cattcattaa ttgtgatctt attcacaatc ttcgtatggt ctggtgctcc tctcagatca
     4681 gctttcatgt tttttgttcg agatgctctc acgtgtttac ttttctgttt tgtctgcagt
     4741 accgatggag ttatcgtttt ggatacggaa ttaatagtaa gcttataaat gaaataatag
     4801 ttaaatgtat aaagatatat ttcagaagta tataattgtc cttacattgg ctaatctcta
     4861 cttcacaact cgttcttcat tctgtactga acgactatcc cgatgtattc aacgagaaaa
     4921 acggtttccc ataaactcaa attttgcttc tcttattaca ggtatgcctt aaaatttttt
     4981 tgtatttttt taaatttaaa atttgatatc taatttcagt tgacacaatg actgattctc
     5041 gtcaaataca atacttcctt agtactgtaa caaagtatca agcagcccaa gactcatatt
     5101 caaatgaatt atttgaaagg ttcccgaaga attggggttg cacatcaatt ctgatatttc
     5161 caatcgtttt cgtctattgg tatttcattg attctaattt tgacaagatt tgtgtttctg
     5221 ttctaccatc attttgtttg gctgctggtg aagagctttt tgctaagaat atgttttgga
     5281 aaggtattta aaaattaagg ttcgagattt taaaactgaa aatgctattt ttagagcgtg
     5341 aagcaatgca agcaaagcaa cgactggaaa atgaagaaca ggctgaaagt attactggaa
     5401 gctcccttga aaaactgttt gctggtaata aaccggtttc taacacagag tgagtgatca
     5461 gaattgccat gaaattaccg aaaaaatcta aatttttaat attttcagca aagctaatat
     5521 tgtcaaaaaa tcaagcatca tcagaaatca aaaaccttgt ctccaagatc tttcacccgg
     5581 tacctacgat gtttcgaatt tcatggtaaa ccattgtaca agaaacatat tcattctcgt
     5641 agttaaatta attacagaag tatccacatc aagcatccag aatattccgg gaaaagatca
     5701 ttggattata tcttaggatt ctgaaattga gaacagtagg ttttcttaat tgcgaaatct
     5761 tttgaatgta ttttttccag ctcggtgtga ttttatgcat tcctgcgatt cttctaattg
     5821 tgatatcaat tggattgctg tttattccag ttaaaagaga aacacttcat acagagtatg
     5881 gtctaatttt tagtataaaa atataattgt gctcttatag ttcgaagcaa gacgacatat
     5941 tcattgagtt cgagattttc aatttttcta ctaattggaa gatagtcaac caaaatctga
     6001 agcagttcag tgaggacatt ggtgagagtt aaatatttga atattctata aattgttcta
     6061 ctttttcaga aagtattgga acactttata caatttcgaa ttggcagaag agtttcgaac
     6121 gatttgaaca agaaactaat aaggtaattc tttccaaaag aagatttgac aacgaaaaaa
     6181 gtgcgaaaag taggattaag tgcgtgtaga ctgtttggaa catttttcgt cttttcgtct
     6241 cccatgattg tctttcgaag aaaaaaaacg ggagaggtta ctgtattctt tctacttttt
     6301 ttcagaatgc ttctgcagaa tggaatatat tgttcaaatg gataaatgat gaaccgatca
     6361 attcagcagt gactttattt tcggaaaaat catctggaaa tcaaacaatt gcaaatccat
     6421 ttaagtttcg tctacgctat gggtttgatg cgaaaaatga gacaactgtg atagaaattg
     6481 ttcaggtgag attttactca aaatttgtga ttttggcatt aaaaatactg tatctggtct
     6541 cactgcgaag tttttttaat gcaaaggggc gtgcgccatc aaagagtact gtaatttccg
     6601 aataaactaa attaatttcc aaaataatta aatttcaaag aaaaatgttt tacgaagcaa
     6661 tgaaaattca acaggttcga aaagttggaa attacagtac tctctaaagg cacagacctt
     6721 ttggcattca acaaaaattg tcctgttgag accaggtaca gtagtttttt aacgaacatt
     6781 cgcaattggt aaataactaa tgtatcaatt cccatgaaag tgttttcaga aaatcgatga
     6841 attgctctcc aaatgctcga aaaacctttc accaaaagca gtcggtgtcc tctacgagca
     6901 ttatcatcga atcgcagttg tctggtgagt ctcagtcggc cacaatcgaa ttgtgtcctt
     6961 tttaatttaa ttttcaaagc ctggaggcag gcattttctg tgtaacaaga attactcctt
     7021 ttttcgaaac gttctgcttt tacacaaatt ggccaccagt gtcttactgt aggtccatct
     7081 tcttatcgaa ttcaaactaa ttatttggca tccaaataat tcatgttatc aagagttttc
     7141 ttctcgtttt ggcattttag gagcagcatt tttgtttccg aaaatttacc gccacccagt
     7201 tagcatgtac tgattaattt ccaacagcag aaagtttaag ttttgagatt tttaagtgct
     7261 ttttctattt ctgaaacttt tcttagatat cctctcctaa agtgcattca ttttctctat
     7321 tttttctcat taatcgcgta ttttaatcat ttctctgaga aaaagttcaa atgcacttta
     7381 tcggaagttg ctcatcaaaa tccgacacct cttcccagca cttgctcttt ttgcttatgt
     7441 gtgtgcgtgt gagtcgaaag ctgctaagaa gacgtgagcc tatgtgctcc gggcggtcct
     7501 cctagtcgcg ccggcgccac caccgagttc gcgaaagtaa gaagacgacg aaaaccaaaa
     7561 gaaaaaggac aaaaaatagg atgaaaatga aatagaaagg acggaagaga taacagaaac
     7621 aaatgattga ttgacaggga caaatagaca gacttctgtt cgaaaatctt tgggttgagg
     7681 gaggtcagaa aaacaaatat ctggagctca atgaatcagc gcggcgttct tcaagatttg
     7741 gatctgaatc tgaatatcca gaacctaccc acccgcgtca taatctttct gatcttaaac
     7801 ctgggttcct gctcactcaa aatgtataac ctagatcgtg cattttgcaa attttctttg
     7861 aaacaatttt gacaatagat ttttatttat attctaatgg aattctgatc tcaaaatatt
     7921 tgtagagttt ttgtttttct aaatattatt tctagcatta cataacccag cgccacatat
     7981 acggtatccg gtctcgacac gataatattt tgttaaatac aaaaagtgtg tgcatttaaa
     8041 gattactgca gtttcaaact ttcaaaatcg aataaaaaac tgacatttta ttcgatatcc
     8101 ataatttttt ctcacaattt cttgaaattc atcttctgtt catctccaat gtctatcgtg
     8161 acaaaaatcc agaaaagttc agttctatga ttaatccttt ttctcaagtg attaacatgc
     8221 caatgtgcga aagttctcta aatccccaga actttgaagt tccatttttt tctgaaaact
     8281 ttgagacttg ttccacagaa tctaaactca atcggaagtg tttcttagca attcatcttt
     8341 tgaatgtgtg gtaccaccat gtgaggcatt cttcaaagtg tgctccgccc tctttttctc
     8401 cctcaatgtc ctttttacac actgggcgac ggcgacggcg gcgggcccta ggatagacgc
     8461 gaagaaaata atttcatctt cgtcatcacc gttgcttcta cttcttttcc tttcccatgg
     8521 gctttcagat gtgttggaac aaagttgttg tcctcctgtg ttttatgttc atgattttct
     8581 cttctaaata ttaagtaaac cccctaaaat agatgaaaga ggaggaagat ggcaattgtt
     8641 tcccctttca tttacatcac tgtcactttt ccgacccctc tgccgataat gatcaagaaa
     8701 tgggggaaat tactggaaaa aaacggtttg atggaaagtg taaagaatct tggaggtttc
     8761 tatcgtttct cgtgataacg aaaaagtgtt acagtagtgt gggcgggatt aatggttcaa
     8821 taaacaactt gaaaccctta gtaaccaaat atctaatgac aaaactattg atattttaat
     8881 ttgaggactg taggcctaca gtaatctttt tggttttcaa atagtttaca caaagcacct
     8941 ccagcttttt cactgataat ataaaaattt catgatcaaa agacaatttg acctccacat
     9001 tttttataaa catcagaacc tccattcctg tttaattttg acctactgat ctagctctca
     9061 atatgttttg ttcaggaatc tcttcgcttt caatcaactc actacagcag ggatcttcat
     9121 tattctgttg tctataatca ctttcatctt tgccatcaca ccaactatca aggcgacttt
     9181 tctggttagt taataataca actttaaaag atttaattca aaattttagt tttccttatt
     9241 ggtcgttggg acgcaaatcg aagtggctgc attggtccac ttattctcac tcgatcacca
     9301 ccaaatctac actaatttgg cactttttgc tggtaaacat aaatatgaaa attttaaatt
     9361 ttaaactcgt ttttcaggtt tcctcgccgc ctgggatccg ttttgtgctt tattgcgata
     9421 tcgaagacga attttgtaca aaagtgaaac tagacgaacc cctgagttgg catcaaagag
     9481 acgagttctt ttgccaattg ttgctacagc tgatattgct caattttttg tgttacttat
     9541 tacaggtatg atttagattc agttttgtgt aaactaaaaa tgtaaattaa ataataacac
     9601 taagaaatat agggcaatct agagcaatgg tgagtggcaa ttattatctg caattctacc
     9661 aaatggcata ttattgcacc atgtttgaaa attagtgtta aactatagaa aatcacttaa
     9721 tttatctgaa aataacattt atttcagttg aaacatttga aaagtgcata aacattacct
     9781 gaaagttgca aattgcctat catcgatctt ttttcagcaa tcggcaactt tcgagccatt
     9841 tttgcatttt ctaagacgtt tcaactgaaa cacattttat ttttagattg aatttttttt
     9901 ctatattttc gcagtaattt tttaattaaa aaaaaatgtt agaaaaacat ggtgcaatgg
     9961 gacattttgt agattttccg aagatcaggt tgatctatta gtaaggattg agttttcctc
    10021 ccctccctct taatttttct ttttaaagaa tttccgaagc gtgaagtaac ttgtcaaaat
    10081 tggtggccat tcagcagttc tcggtcatca atttgttcat ttttcttcca ctcacatccg
    10141 tcatccatta ttcttgccac cttgtccatt tttcgccaat atatatattt attctcactt
    10201 ttctctttct ctcattcatc ttccgacgtt tcaaataaga agtcccgagg ttactgtaga
    10261 tgtatgagtt gcttttcaat cttttttcaa cgtgtgtggc ctcaagagca ttggaacacg
    10321 gccaacaatg aaaaagggac acaaaaagaa agtggctgct agctttggat agagacctca
    10381 tttatcactt tattctcttg aggaattcaa tgaatatgtg aaacttgaat aaatgttctg
    10441 aattctttga tttttgaaca ttataaaaca cacatagact aaatagaatt caccgttctc
    10501 attggattcc agaattctac cgtaaccatg aaaatgtcaa atgactgatt agttaactac
    10561 tataactctg caagtttcat tgaactctca tttcattttt catattacaa atttcaaaaa
    10621 tatatatcta ggatttataa aagtttagac tatgttcatt cgttcctaat aaagtacatt
    10681 tgaagtcggg cgtaaagcac aaacaatttt tagctgtcac aacccactct caaccctact
    10741 gcctttcctt tcttcttctc tctcgtctat gctccgccct ttccgtcggt tttcttcgtc
    10801 gtagttctag tgtctcgaca tggtggcatt cgttccttga aaagaaaaag aatgtttcgt
    10861 attcatgaga cgagagaaac tgaagtaaca aaaaaagggg tatgaactcg aaaaaagaag
    10921 gaaaaggaga ccgaaaatga attgatggtg attaggagct gtgggggttg tgtcttggaa
    10981 gaggaaaaaa ccagttgaaa atgaagctcc aggagagatg aatataacgc agaagtgaaa
    11041 ttttgcgttt tttgctccaa aaatacagta cttggacttg gcacgataaa tatttcctac
    11101 atgcgaacag atgtgcggct ttgaatatta ctgtaatttg aaactcgttg ctggtttaag
    11161 ggaaaattcg cagcagggaa ggtttgaagt tacagtactc ctcgaaatta cagtacgcgc
    11221 acacatgttc gcatttaact caaaattgac cgtgtcgaga ccgggtaccg tatttttagt
    11281 gcaaatattg ctaatgtttg tatctttgta atagaaaaat tgtaagaaat tacaataact
    11341 tgaaatttca atctttgcaa aatagtaatt tttaaatatg tatattttca gcgttctcca
    11401 tacttgcgat catctgctcc atcgttcccg agctcaatat attcttcgta cctacagtaa
    11461 tcctcattgt cattcaaatt gtcgctgtgt tcaattcaat catcgtttca attgcaacaa
    11521 aacaaatgtt tgaaagtgaa gtaagtatgg attccttttc aatattaagc ccatcagatt
    11581 tggtcgtcta agtcgggatt atattcagtt tctctggcag cagcagtagt tcttctttta
    11641 taatctcatc ttcattctaa tattctcaca ttcttttcag gttcgacact atcttcatag
    11701 agacttgcgg ggcagcacta ccgctgtccg ggtctacaat ttggtgcaga aacaaagact
    11761 tgcttcatcg ttagacgagc ctcaggtcga gctggacgaa ttctcaatca aacgatcatc
    11821 tcctccctgc cgatattatg ctccaccacc aaaatattca tgtaagaaaa gaagtcgatc
    11881 atctgatgaa gacgaggatt ctgacccaaa tcagccagga ccatccaatc gtagaagccc
    11941 caaaaccggc aataaacgtg tcagaggaaa tggtgataac actgaacttt atattccaaa
    12001 tcgttatgag ctcattgttt ctggaaagtc agtaggcggt aacactagtg ctgcatggaa
    12061 tggggtaagc tatcatttcc atcaaaactg ttatttttga aacaaatttt cagcctggaa
    12121 gcactttgga acaaaatatg aatgctcttg aggaatgctt cgaattggga gttgatgagt
    12181 atgattttga cgagcatgat ggggatgaag gttgcgaact ggttcaagat atgctagatc
    12241 gagaaagaaa tttgatgaat aaaagatcta cggcacagag aagggaatct cgaaacattg
    12301 aaaagatgaa gaagtcgcaa gaaaatttgg acaaagaaaa gagtgaagag aagatcagtg
    12361 aatcgaagaa gaatcaggat gacagcatag agagtccgaa tctcccagga acacctgcca
    12421 accttccagt tgatgaacca cttccaccag ttggtcgatt gtatattgtg gagcatgtcc
    12481 ttccagaaga atatcggcga gatccattaa ccgagccacc atcaatggaa gattgcattc
    12541 gagctcactc tgaccccaac cttccacctc atccgcgtgc tgatcaatac ccagcctcat
    12601 tcacccgtcc aatggttgaa tattgcgaag atatttactg gacacaccga actggacagc
    12661 tacctccagg acttcaagta ccccgtcgac cgtacgatta ttatcacatt accgagcgta
    12721 caccaccacc ggaagatctc aactgggtcc caccagccga atccccaccg atcccgattc
    12781 ctcagcaagc attcgatttg ctagaagaaa gaagacgaaa tcatcgtgag caacaagatg
    12841 aagcacggga aggcgaccta tcagacccag aggtttcggt accggtagaa aaaatgagta
    12901 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
    12961 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
    13021 cccttaaatt tatttgcact actggaaaac tacctgttcc atgggtaagt ttaaacatat
    13081 atatactaac taaccctgat tatttaaatt ttcagccaac acttgtcact actttctgtt
    13141 atggtgttca atgcttctcg agatacccag atcatatgaa acggcatgac tttttcaaga
    13201 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
    13261 acaagacacg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
    13321 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
    13381 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
    13441 atgtatacat catggcagac aaacaaaaga atggaatcaa agttgtaagt ttaaacatga
    13501 ttttactaac taactaatct gatttaaatt ttcagaactt caaaattaga cacaacattg
    13561 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
    13621 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
    13681 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
    13741 gcatggatga actatacaaa tagcattcgt agaattgtaa gtttaaactt tctcatacta
    13801 actaacgcgc tctatttaaa ttttcagaat tcaatgtctg tttccttttt cagaatttgg
    13861 ttccaaaatt tcatttctta ctcattttcg tttctattat ttgtacaatt tccatttcat
    13921 tatttaattt cttatctact catatctact catatttaat ttcttatcta ctcatatcta
    13981 atcgtccact cgacctcaac ttgtaatctt tacgaatttt gttttcttgt taataaacgt
    14041 aataagttta aaacatttat tataaatcag caatacttaa atgctctggg cgtcaaaaca
    14101 atgacaaatt tgaattattt tcaaaaatta ggaagtgaga aagagcacgg acctgccggt
    14161 ggattccaac atattgaaac gatgagaaag atgaaggaat tgtggaataa tgtaacaaaa
    14221 ttatattcta ccggacaatg aaaaagtgag agaacctgca ctaatattta aatatttgga
    14281 agggggaagg ctattgaaaa atgcaatgaa atgagtgctt aaaattgaga tgcgctgggt
    14341 ggaagtgcga tacagtttat gaaaaagtat taagattaag gcaaaaacat ggaaggaggg
    14401 gcgaattggt aggtgaaaag gcacaaacaa ttataggggg attattataa tttatattac
    14461 aaaaaggatt tgaaaatagg atacgatgga atttaaatac aacgaggcgt ttgaatagct
    14521 tatcaatata ttttctatat tttcgagaga ttttatatac ttaaaagtaa gatttaatgc
    14581 cggtcatttt gaggacgaga ggaatgttag tgatggaaaa taggaaggta atatattttt
    14641 gcaactaaat tcttccaatt tgtcaagcga taaactagtc ggccgtacgg gccctttcgt
    14701 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    14761 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    14821 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
    14881 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
    14941 ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    15001 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    15061 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    15121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    15181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    15241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    15301 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    15361 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    15421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    15481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    15541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    15601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    15661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    15721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    15781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    15841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    15901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    15961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    16021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    16081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    16141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    16201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    16261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    16321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    16381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    16441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    16501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    16561 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    16621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    16681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    16741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    16801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    16861 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    16921 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    16981 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    17041 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    17101 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    17161 ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta
    17221 aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg
    17281 gaaatgaaat
//
