LOCUS       sequence-128370-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses zebrafish Chk1 mutant with C-term GFP fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Zhang M, Kothari P, Mullins M, Lampson MA
  TITLE     Regulation of zygotic genome activation and DNA damage checkpoint 
            acquisition at the mid-blastula transition.
  JOURNAL   Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066.
  PUBMED    25558827
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2014"; volume: "13(24):3828-38. doi"; pages: " 
            10.4161/15384101.2014.967066"
COMMENT     Sequence Label: pMZ031
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc gaattcagga
      121 ccatggctgt gccttttgtt aaagactggg atgtggtaca aactcttgga gagggagcat
      181 atggagaggt gcgactgctg gtcaacaaga aaacagaaga ggctgtggcg gtgaaagttg
      241 tggacatggc aaaagccaag gattgcatcg agaatgtgaa gaaggaggtc tgcatatgca
      301 agatgctttc acaccccaac attgtacgtt tctttgggca cagtgttggg attacacatc
      361 gtgacataaa gcctgagaat attcttcttg atgataaaga taatctgaag atctctgact
      421 ttggcctggc taccatgttc aggcaccgtg gccgtgagcg agctttgaac cgtctgtgtg
      481 gtactctgcc ctatgttgcc ccagagttga tgtcacgctc atcttttaac gctcagcctg
      541 cggacacttg ggcttgtggc attgtgctca ctgcaatgtt agctggagag ttaccctggg
      601 atcagccgag tgaaaactgt caggaatatt tggactggct ggaaagaaag acctacctta
      661 caccctggaa gaaaattgat gcggtacccc ttagtctgtt gtctaagata ttactgcaca
      721 atccagaaga caggttcacc attcctgaaa ttaagaaaca ccgctggttt agcagaagtt
      781 tcaaatcagc agtacaacgt cagggcatca caccagcagc caagattcaa cgggctgact
      841 ctgagctgag acgaaa
//
