LOCUS       sequence-128381-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Cas9 protein and puromycin 
            resistance from EFS promoter and a DDX3Y targeting synthetic 
            single-guide RNA (sgRNA) element from U6 promoter. Lentiviral 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: lentiCRISPR - DDX3Y sgRNA 2
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        220..295
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    343..460
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgt cttgttgggg ctaaaaccag ttttagagct agaaatagca
      241 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      301 tgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
      361 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      421 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      481 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
      541 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
      601 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ct
//
