LOCUS       sequence-128386-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal domain of Chk1 with C-term GFP fluorescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Zhang M, Kothari P, Mullins M, Lampson MA
  TITLE     Regulation of zygotic genome activation and DNA damage checkpoint 
            acquisition at the mid-blastula transition.
  JOURNAL   Cell Cycle. 2014;13(24):3828-38. doi: 10.4161/15384101.2014.967066.
  PUBMED    25558827
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2014"; volume: "13(24):3828-38. doi"; pages: " 
            10.4161/15384101.2014.967066"
COMMENT     Sequence Label: pMZ040
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(763..784)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaggac
      121 cattggttta gcagaagttt caaatcagca gtacaatgtc agggcatcac accagcagcc
      181 aagattcaac gggctgactc tgagctgaga cgaaaaaaca gtgatgaccg agcgcagatc
      241 tccagctctc agcctgagcc acagggatta tgggaggaga aggaggttac agttcacacc
      301 gatgtcagtg tcagcttttc acaaccgacc tgtcctgacc acatgctgct gggcacacaa
      361 ctgcttggca caccgggtgc aagccagagc ccctggcagc gtttggtgag gagaatgacc
      421 aggttcttta ctacagaaaa tacagaggca tcttgcattg ctctccgtga ttcttgtatt
      481 gctttgggtc acacatggaa acagagctgt aataatcagg cgacagtgtc tacaatggat
      541 cgtcgcaata ataagctgat attcatagtg cacttcctgg aaatggagga acgtatttta
      601 gttgatttcc ggctgtcaaa gggggacggt ttggaattca agcgaatatt cctgaaactc
      661 aaacagaagc tgtctgacat tattagcaac caaaaggttt tgccattgat tctcgagatg
      721 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      781 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      841 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc c
//
