LOCUS       sequence-128410-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fibrillarin nucleolar marker with TurboRFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pTagRFP-C1-Fibrillarin
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..762
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtgccac catggtgtct
       61 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtatatgga gggcaccgtg
      121 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      181 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
      241 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
      301 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
      361 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      421 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
      481 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
      541 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
      601 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
      661 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
      721 agatactgcg acctccctag caaactgggg cacaaactta attctcgagc tcaagcttcg
      781 aattctgcag tcgacggtac catgaagcca ggattcagtc cccgtggggg tggctttggc
      841 ggccgagggg gctttggtga ccgtggtggt cgtggaggcc gagggggctt tggcgggggc
      901 cgaggtcgag gcggaggctt tagaggtcgt ggacgaggag gaggtggagg cggcggcggc
      961 ggtggaggag gaggaagagg tggtggaggc ttccattctg gtggcaaccg gggtcgtggt
     1021 cggggaggaa aaagaggaaa c
//
