LOCUS       sequence-128412-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_omega1R
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..41
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     162..181
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     375..397
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     389..406
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     390..406
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    407..463
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(476..492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(476..492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(489..511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    500..516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(524..530,531..548,549..554))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    569..590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    678..702
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cgctcttttc tcttaggttt acccgccaat atatcctgtc agaattcggt ctcatgacgg
       61 agtgagacgc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      121 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
      181 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
      241 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggcgcaggc
      301 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      361 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg
      421 tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat
      481 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      541 gcatatagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      601 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcgtctc acgctctcct
      661 gagaccgaat tcgttatgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
      721 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
      781 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
      841 ggaagaacgg
//
