LOCUS       sequence-128413-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fibrillarin nucleolar marker with TurboRFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pTagRFP-C1-Fibrillarin
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             313..324
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cctcagttct tcaccttggg gggtggcctg tacactccca cgaccacggc
      181 atggtctctt tcatatggct caagggtcaa ctgctcctgc ggcttcatgt tctcctgttg
      241 catctttttc acttcggagg caaacacggc ctcggctgag gctgtggagt caatgcagtt
      301 ggccttaatg gaaatcacaa agtgtcctcc attacgcagg aaggtgtggg cattcagggc
      361 cacaatccgg gtctggtctg gctgggccac atcagcaaag atcacatcca ccattgcgat
      421 gagcatgcgg tatttgtgtg ggtgtcgagc atcctcgatc acaggaatga tgttggtcct
      481 cttcttggcc aagttaatga ggtcacggcc agagcggtgg gagaactcga ctgcatagac
      541 tagaccatcc ggaccaacga tgtcagagac atgggagacc gtggtgcccg aggcagcccc
      601 gaggtagaga accttagccc ccggtttgat gtggatctgg tccacaccac ccaggattgc
      661 tgctgctagc ttggagcgga aggggttcca ggctcggtac tcaattttgt catctccttc
      721 cgaaatcgag actctcttct ctccataaac tgattcccca gggaccaggt tcttggtgac
      781 cagtgcatct tcctttcctc gacaaatgaa gacaccctca tgccgatgcg gctccaccat
      841 cacattcttc cccgactggt ttcctctttt tcctccccga ccacgacccc ggttgccacc
      901 agaatggaag cctccaccac ctcttcctcc tcctccaccg ccgccgccgc ctccacctcc
      961 tcctcgtcca cgacctctaa agcctccgcc tcgacctcgg cccccgccaa agccccctcg
     1021 gcctccacga ccaccacggt caccaaagcc ccctcggcgc
//
