LOCUS       sequence-128424-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of mCherry with full-length p63RhoGEF in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   van Unen J, Reinhard NR, Yin T, Wu YI, Postma M, Gadella TW, 
            Goedhart J
  TITLE     Plasma membrane restricted RhoGEF activity is sufficient for 
            RhoA-mediated actin polymerization.
  JOURNAL   Sci Rep. 2015 Oct 5;5:14693. doi: 10.1038/srep14693.
  PUBMED    26435194
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-5"; volume: "5:14693. doi"; pages: " 
            10.1038/srep14693"
COMMENT     Sequence Label: pmCherry-C1-p63RhoGEF
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..331
                     /codon_start=1
                     /product="palmitoylation signal consisting of the first 29 
                     amino acids of p63RhoGEF (Goedhart et al., 2013)"
                     /label=palmitoylation signal
                     /translation="RGGHKGGRCACPRVIRKVLAKCGCCFAR"
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagtcccgg ggggggcaca aagggggtcg ctgtgcctgt ccccgtgtga tccgaaaagt
      301 gctggcaaaa tgcggctgct gcttcgcccg ggggggacgt gaatcctatt ccattgcggg
      361 cagtgagggg agtatatcgg cttctgctgc ctccggtctg gctgccccct ctggccccag
      421 ctctggcctc agctctggcc cctgttcccc aggcccccca gggcccgtca gtggcctgag
      481 gagatggttg gatcattcca aacattgtct cagtgtggaa actgaggcag acagtggtca
      541 ggcaggacca tatgagaact ggatgttgga gccagctcta gccacaggag aggagctgcc
      601 ggaactgacc ttgctgacca cactgttgga gggccctgga gataagacgc agccacctga
      661 agaggagact ttgtcccaag cccctgagag tgaggaggaa cagaagaaga aggctctgga
      721 aaggagtatg tatgtcctga gtgaactggt agaaacagag aaaatgtacg tggacga
//
