LOCUS       sequence-128434-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(301..321)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcgccggag cctgaagggc gtactagggt tgcgaggtcc aatgcattaa tgcattgcag
       61 atgagctgta tctggaagag gtaaacccga aacgcgtttt attcttgttg acatggagct
      121 attaaatcac tagaaggcac tctttgctgc ttggacaaat gaacgtatct tatcgagatc
      181 ctgaacacca tttgtctcaa ctccggagct gacatcgaca ccaacgatct tatatccaga
      241 ttcgtcaagc tgtttgatga tttcagtaac gttaagtacg cgtctcgagg tccaaaatca
      301 caccttcctc ttcttcttgg ggtcagccct gctgtctcca ccgagctgag agaggtcgat
      361 tcttgtttca tagagccccg taattgactg atgaatcagt gtggcgtcca ggacctcctt
      421 tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag tacttgaagg ctgcaggcgc
      481 gcccaagttg gtcagagtaa acaagtggat aatgttttct gcctgctccc tgatgggctt
      541 atccctgtgc ttattgtaag cagaaagcac cttatcgagg ttagcgtcgg cgaggatcac
      601 tcttttggag aattcgctta tttgctcgat gatctcatca aggtagtgtt tgtgttgttc
      661 cacgaacagc tgcttctgct cattatcttc gggagaccct ttgagctttt catagtggct
      721 ggccagatac aagaaattaa cgtatttaga gggcagtgcc agctcgttac ctttctgcag
      781 ctcgcccgca ctagcgagca ttcgtttccg gccgttttca agctcaaaga gagagtactt
      841 gggaagctta atgatgaggt cttttttgac ctctttatat cctttcgcct cgagaaagtc
      901 gatggg
//
