LOCUS       sequence-128435-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    56..559
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     601..619
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(657..679)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     779..798
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
       61 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
      121 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
      181 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
      241 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
      301 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
      361 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
      421 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
      481 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
      541 aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa
      601 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
      661 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      721 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      781 gcatcagagc agattgtact gagagtgcac catatcgact acgtcgttaa ggccgtttct
      841 gacagagtaa aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt
      901 gacttaaact ccatcaaatg gtcaggtcat tgagtgtttt
//
