LOCUS       sequence-128439-        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        425..832
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatcga
      181 ccggtcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
      241 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
      301 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
      361 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
      421 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
      481 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
      541 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
      601 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
      661 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
      721 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
      781 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
      841 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
      901 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
      961 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     1021 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     1081 taccggtagt gttagacctg aacaaggttt actaaaa
//
