LOCUS       sequence-128446-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 1, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai1-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(564..585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(825..844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc
      121 actgtgctgg atatctgcag aattccatgg gctgcacact gagcgctgag gacaaggcgg
      181 ccgtggagcg cagcaagatg atcgaccgca acctccggga ggacggagag aaggcagcgc
      241 gcgaggtcaa gctgctgctg ctgggtgctg gtgaatccgg gaagagcaca attgtgaagc
      301 agatgaaaat tatccacgag gctggctact cagaggaaga gtgtaagcag tacaaagcag
      361 tggtctacag caacaccatc cagtccatca ttgccatcat tagagctatg gggagattga
      421 aaatcgactt tggagacgct gctcgtgcgg atgatgctcg ccaactcttc gtgcttgctg
      481 gggctgcaga ggaaggcttt atgaccgcga ccggttctat ggtgagcaag ggcgaggagc
      541 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      601 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      661 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgtcctggg
      721 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      781 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      841 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      901 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagt
//
