LOCUS       sequence-128448-         305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB333
FEATURES             Location/Qualifiers
     source          1..305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(24..30,31..48,49..54))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(91..215)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
       61 ggtgcctaat gcggcccctc gaggggccgc catagtgact ggatatgttg tgttttacag
      121 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      181 tacgtttctc gttcagcttt tttgtacaaa cttgttgata actctagatg gtggaacttc
      241 gctctcgact gcaggaagat cagacagaac tcaacaattc ggtgtcgatc tcggatggga
      301 acgtg
//
