LOCUS       sequence-128457-       11249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 11249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..11249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2755..2775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2789..3146
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3170..7273
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3170..7273
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3170..7273
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7286..7306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7478..8045
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(8138..8157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8139..8157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8167..8184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8167..8183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8176..8198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8328..8783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8415..8434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8625..8646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9084..9491
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9492..10586
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11080..11099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11199..11221
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag aagatgatat tgaaggagca tttttgggct
     2821 tggctggagc tagtggaggt caacaatgaa tgcctatttt ggtttagtcg tccaggcggt
     2881 gagcacaaaa tttgtgtcgt ttgacaagat ggttcattta ggcaactggt cagatcatcc
     2941 ccacttgtag cagtagcggc ggcgctcgaa gtgtgactct tattagcaga caggaacgag
     3001 gacattatta tcatctgctg cttggtgcac gataacttgg tgcgtttgtc aagcaaggta
     3061 agtgaacgac ccggtcatac cttcttaagt tcgcccttcc tccctttatt tcagattcaa
     3121 tctgacttac ctattctacc gaagcactag tggatccccc gggaaaaaaa tggacaagaa
     3181 gtactccatt gggctcgata tcggcacaaa cagcgtcggt tgggccgtca ttacggacga
     3241 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     3301 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     3361 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     3421 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     3481 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     3541 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     3601 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     3661 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     3721 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     3781 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     3841 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     3901 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     3961 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     4021 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     4081 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     4141 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     4201 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     4261 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     4321 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     4381 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     4441 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     4501 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     4561 gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa
     4621 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     4681 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     4741 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     4801 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     4861 caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     4921 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     4981 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     5041 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     5101 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     5161 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     5221 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     5281 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat
     5341 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     5401 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     5461 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     5521 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     5581 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     5641 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
     5701 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
     5761 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     5821 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     5881 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     5941 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     6001 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc
     6061 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     6121 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct
     6181 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     6241 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     6301 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga
     6361 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     6421 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     6481 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     6541 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     6601 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     6661 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     6721 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     6781 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     6841 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     6901 gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     6961 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     7021 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     7081 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     7141 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     7201 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     7261 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgtgat tttggacctc
     7321 gagacgcgta cttaacgtta ctgaaatcat caaacagctt gacgaatctg gatataagat
     7381 cgttggtgtc gatgtcagct ccggagttga gacaaatggt gttcaggatc tcgataagat
     7441 acgttcattt gtccaagcag caaagagtgc cttctagtga tttaatagct ccatgtcaac
     7501 aagaataaaa cgcgtttcgg gtttacctct tccagataca gctcatctgc aatgcattaa
     7561 tgcattggac ctcgcaaccc tagtacgccc ttcaggctcc ggcgaagcag aagaatagct
     7621 tagcagagtc tattttcatt ttcgggagac gagatcaagc agatcaacgg tcgtcaagag
     7681 acctacgaga ctgaggaatc cgctcttggc tccacgcgac tatatatttg tctctaattg
     7741 tactttgaca tgctcctctt ctttactctg atagcttgac tatgaaaatt ccgtcaccag
     7801 cccctgggtt cgcaaagata attgcactgt ttcttccttg aactctcaag cctacaggac
     7861 acacattcat cgtaggtata aacctcgaaa atcattccta ctaagatggg tatacaatag
     7921 taaccatggt tgcctagtga atgctccgta acacccaata cgccggccga aactttttta
     7981 caactctcct atgagtcgtt tacccagaat gcacaggtac acttgtttag aggtaatcct
     8041 tctttctaga gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
     8101 gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt cgtattacgc
     8161 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8221 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8281 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
     8341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8401 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8521 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8641 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8701 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8761 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     8821 atttcacacc gcatatcgac cggtcgagga gaacttctag tatatctaca tacctaatat
     8881 tattgcctta ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa
     8941 tcaattgtcc tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat
     9001 tatactctat ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt
     9061 caagaaatat cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag
     9121 ttcgaatctc ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc
     9181 tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga
     9241 cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac
     9301 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga
     9361 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt
     9421 actttctaac ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat
     9481 ataccattct aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc
     9541 aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg
     9601 tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc
     9661 cacttccaga tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg
     9721 tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta ctaaaaatcc
     9781 gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt
     9841 tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag
     9901 aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt
     9961 gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca
    10021 tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg
    10081 cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa ttccctacat
    10141 tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc
    10201 acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct
    10261 ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca
    10321 agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga
    10381 ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga
    10441 acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta
    10501 tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg
    10561 aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa
    10621 actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat
    10681 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
    10741 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
    10801 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
    10861 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
    10921 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
    10981 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
    11041 aacggcctta acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc
    11101 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    11161 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    11221 gttttcaccg tcatcaccga aacgcgcga
//
