LOCUS       sequence-128467-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of MKLP1 with an eGFP fusion and an S708A 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Douglas ME, Davies T, Joseph N, Mishima M
  TITLE     Aurora B and 14-3-3 coordinately regulate clustering of 
            centralspindlin during cytokinesis.
  JOURNAL   Curr Biol. 2010 May 25;20(10):927-33. doi: 
            10.1016/j.cub.2010.03.055. Epub 2010 May 6.
  PUBMED    20451386
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2010.03.055"; journalName: "Curr Biol"; date: 
            "2010-05-25- 25"; volume: "20"; issue: "10"; pages: "927-33"
COMMENT     Sequence Label: pEGFP-C1-MKLP1 S708A
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     289..310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
       61 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      121 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      241 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact
      361 cagatctcga gctctgggca tggggaagtc agcgagagct aagacacccc ggaaacctac
      421 cgtgaaaaaa gggtcccaaa cgaaccttaa agacccagtt ggggtatact gtagggtgcg
      481 cccactgggc tttcctgatc aagagtgttg catagaagtg atcaataata caactgttca
      541 gcttcatact cctgagggct acagactcaa ccgaaatgga gactataagg agactcagta
      601 ttcatttaaa caagtatttg gcactcacac cacccagaag gaactctttg atgttgtggc
      661 taatcccttg gtcaatgacc tcattcatgg caaaaatggt cttcttttta catatggtgt
      721 gacgggaagt ggaaaaactc acacaatgac tgg
//
