LOCUS       sequence-128483-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    247..294
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tgctgaagga agcatacgat accccgcatg gaatgggata atatcacagg aggtactaga
       61 ctacctttca tcctacataa atagacgcat ataagtacgc atttaagcat aaacacgcac
      121 tatgccgttc ttctcatgta tatatatata caggcaacac gcagatatag gtgcgacgtg
      181 aacagtgagc tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa
      241 cgctttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg
      301 cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac
      361 tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct
      421 ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct
      481 tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt
      541 agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt
      601 aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg
      661 accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc
      721 ggtatagaat ataatc
//
