LOCUS       sequence-128486-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..151)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(159..175)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..175)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(190..631)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(196..219)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    514..631
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(655..675)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(655..673)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(694..710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(707..729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    718..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(742..748,749..766,767..772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
       61 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      121 tttctcgttc agcttttttg tacaaacttg tgatggggga tccactagtt ctagaatccg
      181 gggttttttc tccttgacgt taaagtatag aggtatatta acaatttttt gttgatactt
      241 ttattacatt tgaataagaa gtaatacaaa ccgaaaatgt tgaaagtatt agttaaagtg
      301 gttatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg cttcgctgat
      361 taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg gttgttaatt
      421 tgattcgttc atttgaaggt ttgtggggcc aggttactgc caatttttcc tcttcataac
      481 cataaaagct agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct
      541 gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg aggagagtct tccttcggag
      601 ggctgtcacc cgctcggcgg cttctaatcc gtactagagc tccagctttt gttcccttta
      661 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      721 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctg
//
