LOCUS       sequence-128488-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             294..371
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             399..419
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatgg
      241 gctgcaggaa ttcgatatca agcttatcga taggtggacc aggtggtgga atgaagcgac
      301 gatggaaaaa gaatttcata gccgtctcag cagccaaccg ctttaagaaa atctcatcct
      361 ccggggcact tgattatggt accccaacta ctgcttctga aaatctatat tttcaaggtg
      421 aactaaaaac tgctgctttg gctcaacatg cgattaaggg tgaagctcaa aaacttaatg
      481 actctcaagc tccaaaagct gatgcgcaac aaaataactt caacaaagat caacaaagcg
      541 ccttctatga aatcttgaac atgcctaact taaacgaagc gcaacgtaac ggcttcattc
      601 aaagtcttaa agacgaccca agccaaagca ctaacgtttt aggtgaagct aaaaaattaa
      661 acgaatctca agcaccgaaa gctgataaca atttcaacaa agaacaacaa aatgctttct
      721 atgaaatctt gaatatgcct aacttaaacg aagaacaacg caatggtttc atccaaagct
      781 taaaagatga cccaagccaa agtgctaacc tattgtcaga agctaa
//
