LOCUS       sequence-128491-         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..405
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(61..80)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
       61 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      121 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      181 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      241 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      301 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      361 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      421 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      481 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttat
//
