LOCUS       sequence-128503-       10103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for FOXM1c expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Barger CJ, Zhang W, Hillman J, Stablewski AB, Higgins MJ, 
            Vanderhyden BC, Odunsi K, Karpf AR
  TITLE     Genetic determinants of FOXM1 overexpression in epithelial ovarian 
            cancer and functional contribution to cell cycle progression.
  JOURNAL   Oncotarget. 2015 Jul 16.
  PUBMED    26243836
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2015 Jul 16."
COMMENT     Sequence Label: pCW57.1-FOXM1c
FEATURES             Location/Qualifiers
     source          1..10103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      610..619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             619..642
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             1975..1986
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(2976..3000)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3018..3035
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3045..3555
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3474..3492
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3565..4161
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3565..3584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4061..4081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4162..4215
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4216..4833,4834..4839,4840..4875,4876..4878,4879..4914,
                     4915..4917,4918..4953,4954..4962)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5002..5022)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5420..5431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5636..5869
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5941..6062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5978..5997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6032..6051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6102..6237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6237..6308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6309..6377,6378..7169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6527..6546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7340..7928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7829..7848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8082..8099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8216..8237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8252..8257,8258..8275,8276..8282)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8290..8306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8295..8317
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8314..8330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8314..8330
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8349..8369
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8351..8369
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8397..8623
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8624..8804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8851..8976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9469..9702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9887..9931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tcgggaattc gtcgactgga tccggtaccg
      601 aggagatctg ccaccatgga ttacaaggat gacgacgata aggcgatcgc cggcgcgccg
      661 aaaactagcc cccgtcggcc actgattctc aaaagacgga ggctgcccct tcctgttcaa
      721 aatgccccaa gtgaaacatc agaggaggaa cctaagagat cccctgccca acaggagtct
      781 aatcaagcag aggcctccaa ggaagtggca gagtccaact cttgcaagtt tccagctggg
      841 atcaagatta ttaaccaccc caccatgccc aacacgcaag tagtggccat ccccaacaat
      901 gctaatattc acagcatcat cacagcactg actgccaagg gaaaagagag tggcagtagt
      961 gggcccaaca aattcatcct catcagctgt gggggagccc caactcagcc tccaggactc
     1021 cggcctcaaa cccaaaccag ctatgatgcc aaaaggacag aagtgaccct ggagaccttg
     1081 ggaccaaaac ctgcagctag ggatgtgaat cttcctagac cacctggagc cctttgcgag
     1141 cagaaacggg agacctgtgc agatggtgag gcagcaggct gcactatcaa caatagccta
     1201 tccaacatcc agtggcttcg aaagatgagt tctgatggac tgggctcccg cagcatcaag
     1261 caagagatgg aggaaaagga gaattgtcac ctggagcagc gacaggttaa ggttgaggag
     1321 ccttcgagac catcagcgtc ctggcagaac tctgtgtctg agcggccacc ctactcttac
     1381 atggccatga tacaattcgc catcaacagc actgagagga agcgcatgac tttgaaagac
     1441 atctatacgt ggattgagga ccactttccc tactttaagc acattgccaa gccaggctgg
     1501 aagaactcca tccgccacaa cctttccctg cacgacatgt ttgtccggga gacgtctgcc
     1561 aatggcaagg tctccttctg gaccattcac cccagtgcca accgctactt gacattggac
     1621 caggtgttta agccactgga cccagggtct ccacaattgc ccgagcactt ggaatcacag
     1681 cagaaacgac cgaatccaga gctccgccgg aacatgacca tcaaaaccga actccccctg
     1741 ggcgcacggc ggaagatgaa gccactgcta ccacgggtca gctcatacct ggtacctatc
     1801 cagttcccgg tgaaccagtc actggtgttg cagccctcgg tgaaggtgcc attgcccctg
     1861 gcggcttccc tcatgagctc agagcttgcc cgccatagca agcgagtccg cattgccccc
     1921 aaggtgctgc tagctgagga ggggatagct cctctttctt ctgcaggacc agggaaagag
     1981 gagaaactcc tgtttggaga agggttttct cctttgcttc cagttcagac tatcaaggag
     2041 gaagaaatcc agcctgggga ggaaatgcca cacttagcga gacccatcaa agtggagagc
     2101 cctcccttgg aagagtggcc ctccccggcc ccatctttca aagaggaatc atctcactcc
     2161 tgggaggatt cgtcccaatc tcccacccca agacccaaga agtcctacag tgggcttagg
     2221 tccccaaccc ggtgtgtctc ggaaatgctt gtgattcaac acagggagag gagggagagg
     2281 agccggtctc ggaggaaaca gcatctactg cctccctgtg tggatgagcc ggagctgctc
     2341 ttctcagagg ggcccagtac ttcccgctgg gccgcagagc tcccgttccc agcagactcc
     2401 tctgaccctg cctcccagct cagctactcc caggaagtgg gaggaccttt taagacaccc
     2461 attaaggaaa cgctgcccat ctcctccacc ccgagcaaat ctgtcctccc cagaacccct
     2521 gaatcctgga ggctcacgcc cccagccaaa gtagggggac tggatttcag cccagtacaa
     2581 acctcccagg gtgcctctga ccccttgcct gaccccctgg ggctgatgga tctcagcacc
     2641 actcccttgc aaagtgctcc cccccttgaa tcaccgcaaa ggctcctcag ttcagaaccc
     2701 ttagacctca tctccgtccc ctttggcaac tcttctccct cagatataga cgtccccaag
     2761 ccaggctccc cggagccaca ggtttctggc cttgcagcca atcgttctct gacagaaggc
     2821 ctggtcctgg acacaatgaa tgacagcctc agcaagatcc tgctggacat cagctttcct
     2881 ggcctggacg aggacccact gggccctgac aacatcaact ggtcccagtt tattcctgag
     2941 ctacagtaga gcccctcgag gtttaaacgg ccggcaccca gctttcttgt acaaagtggt
     3001 ttagtaatga accggtccac caccaccacc accactaagg atccggggtt ggggttgcgc
     3061 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     3121 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     3181 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     3241 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     3301 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     3361 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     3421 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     3481 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     3541 gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     3601 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     3661 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     3721 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     3781 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     3841 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     3901 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     3961 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4021 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4081 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4141 atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     4201 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     4261 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     4321 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     4381 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     4441 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     4501 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     4561 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     4621 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     4681 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     4741 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     4801 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     4861 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     4921 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     4981 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5041 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5101 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5161 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5221 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5281 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5341 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5401 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5461 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5521 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     5581 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5641 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5701 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5761 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5821 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5881 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6061 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6121 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6181 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     6241 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6781 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6841 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6901 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6961 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7021 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7081 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7141 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7201 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7261 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8101 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8161 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8221 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8281 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8341 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     8401 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     8461 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     8521 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     8581 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8821 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8881 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8941 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9001 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9061 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9121 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9181 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9241 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9301 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9361 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9421 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9481 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9541 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9601 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9661 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9721 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9781 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9841 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9901 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9961 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    10021 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    10081 ccacctccca accccgaggg gac
//
