LOCUS       sequence-128507-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The heterotrimeric G-protein subunit, G-alpha-i isoform 1, tagged 
            with mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   van Unen J, Stumpf AD, Schmid B, Reinhard NR, Hordijk PL, Hoffmann 
            C, Gadella TW Jr, Goedhart J
  TITLE     A New Generation of FRET Sensors for Robust Measurement of Galphai1,
            Galphai2 and Galphai3 Activation Kinetics in Single Cells.
  JOURNAL   PLoS One. 2016 Jan 22;11(1):e0146789. doi: 
            10.1371/journal.pone.0146789. eCollection 2016.
  PUBMED    26799488
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-01-22"; volume: "11(1):e0146789. doi"; pages: " 
            10.1371/journal.pone.0146789. eCollection 2016"
COMMENT     Sequence Label: Galphai1-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(726..743)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    732..843
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcgccggcgt cataaagaga ctgtggaagg acagcggtgt gcaagcctgc ttcaacagat
       61 cccgggagta ccagctgaac gattcggcgg cgtactacct gaatgacttg gacagaatag
      121 cacaaccaaa ttacatccca acccagcagg atgttctcag aactagagtg aaaacgacgg
      181 gaattgtgga aacccacttt actttcaaag atcttcattt taaaatgttt gacgtgggag
      241 gccagagatc agagcggaag aagtggattc actgctttga aggcgtgact gccatcatct
      301 tctgtgtggc cctgagtgac tatgacctgg ttcttgctga ggatgaagaa atgaaccgga
      361 tgcacgaaag catgaagctg ttcgatagca tatgtaacaa caagtggttt acggacacat
      421 ccatcatcct tttcctgaac aagaaggacc tcttcgaaga gaagatcaaa aagagtcccc
      481 tcacgatatg ctatccagaa tatgcaggct caaacacata tgaagaggcg gctgcgtata
      541 tccagtgtca gtttgaagac ctcaataaaa ggaaggacac aaaggaaatt tacacccact
      601 tcacttgcgc cacggatacg aagaatgtgc agtttgtgtt cgatgctgta acggacgtca
      661 tcataaagaa taacctaaaa gactgtggtc tcttctaagc ttaataagtt taaaccgctg
      721 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
      781 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
      841 atcgcattgt ctgagtaggt gtcattc
//
