LOCUS       sequence-128509-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      400..409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(451..472)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(712..731)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgccacca tgaagttaag tcgccagttc
       61 accgtgtttg gcagcgcgat cttctgcgtc gtaatcttct cactctacct gatgctggac
      121 aggggtcact tggactaccc tcggggcccg cgccaggagg gctcctttcc gcagggccag
      181 ctttcaatat tgcaagaaaa gattgaccat ttggagcgtt tgctcgctga gaacaacgag
      241 atcatctcaa atatcagaga ctcagtcatc aacctgagcg agtctgtgga ggacggcccg
      301 cgggggtcac caggcaacgc cagccaaggc tccatccacc tccactcgcc acagttggcc
      361 ctgcaggctg accccagctc cggatcggat ccaccggtcg ccaccatggt gagcaagggc
      421 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      481 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      541 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      601 agctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      661 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      721 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      781 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      841 gccatccacg gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
      901 ttcggcctca actgcaacat cga
//
